[Dicer能有效地将长双链RNA转化为适用于COX - 2基因的小干扰RNA]
[Dicer efficiently converts large dsRNAs into siRNAs suitable for COX-2 gene].
作者信息
Luo Hong, Hu Dong-xu, Chen Ping
机构信息
Department of Respiratory Diseases Second Xiangya Hospital, Central South University, Changsha 410011, China.
出版信息
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Jun;32(3):437-42.
OBJECTIVE
To report the use of Dicer to cleave double-stranded RNA (dsRNAs) into small interference RNAs (D-siRNAs) that can target multiple sites within an mRNA, and to acquire an new method to cure inflammation of the airway and tumor.
METHODS
Using RiboMAX Large Scale RNA Production Systems-SP6 and T7 kit were used to transcribe A549 cell COX-2 DNA into RNA (dsRNAs). We mixed dsRNAs with Dicer in the reaction buffer. We recovered siRNAs using RNA Purification Column.
RESULTS
Dicer efficiently converted double-stranded RNA of COX-2 into small interference RNAs of 21 approximately 23 bp.
CONCLUSION
Dicer efficiently converts double-stranded RNA (dsRNA) into small interference RNAs (D-siRNAs of 21 approximately 23 bp).
目的
报道使用核酸酶Dicer将双链RNA（dsRNAs）切割成可靶向mRNA内多个位点的小干扰RNA（D-siRNAs），并获得一种治疗气道炎症和肿瘤的新方法。
方法
使用RiboMAX大规模RNA生产系统-SP6和T7试剂盒将A549细胞COX-2 DNA转录为RNA（dsRNAs）。我们在反应缓冲液中将dsRNAs与核酸酶Dicer混合。我们使用RNA纯化柱回收了siRNAs。
结果
核酸酶Dicer有效地将COX-2的双链RNA转化为约21至23 bp的小干扰RNA。
结论
核酸酶Dicer有效地将双链RNA（dsRNA）转化为约21至23 bp的小干扰RNA（D-siRNAs）。
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