双链RNA结构对Dicer特异性和效率的贡献。
The contributions of dsRNA structure to Dicer specificity and efficiency.
作者信息
Vermeulen Annaleen, Behlen Linda, Reynolds Angela, Wolfson Alexey, Marshall William S, Karpilow Jon, Khvorova Anastasia
机构信息
Dharmacon Inc., 2650 Crescent Dr., Suite #100, Lafayette, CO 80026, USA.
出版信息
RNA. 2005 May;11(5):674-82. doi: 10.1261/rna.7272305. Epub 2005 Apr 5.
Dicer processes long double-stranded RNA (dsRNA) and pre-microRNAs to generate the functional intermediates (short interfering RNAs and microRNAs) of the RNA interference pathway. Here we identify features of RNA structure that affect Dicer specificity and efficiency. The data presented show that various attributes of the 3' end structure, including overhang length and sequence composition, play a primary role in determining the position of Dicer cleavage in both dsRNA and unimolecular, short hairpin RNA (shRNA). We also demonstrate that siRNA end structure affects overall silencing functionality. Awareness of these new features of Dicer cleavage specificity as it is related to siRNA functionality provides a more detailed understanding of the RNAi mechanism and can shape the development of hairpins with enhanced functionality.
Dicer 对长双链 RNA（dsRNA）和前体微小 RNA 进行加工，以生成 RNA 干扰途径的功能性中间体（小干扰 RNA 和微小 RNA）。在此，我们确定了影响 Dicer 特异性和效率的 RNA 结构特征。所呈现的数据表明，3' 端结构的各种属性，包括悬垂长度和序列组成，在决定 Dicer 对 dsRNA 和单分子短发夹 RNA（shRNA）的切割位置方面起主要作用。我们还证明了小干扰 RNA 的末端结构会影响整体沉默功能。了解与小干扰 RNA 功能相关的 Dicer 切割特异性的这些新特征，能让我们更详细地理解 RNA 干扰机制，并有助于设计出功能增强的发夹结构。
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