Ma Qi-lin, Sun Ming, Yang Tian-lun, Li Yuan-jian, Tang Can-e, Peng Zhen-yu, He Shi-lin, Chen Fang-ping
Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2007 Jun;32(3):485-9.
To determine the effects of Tongxinluo on cell viability and tissue factor (TF) in AngII induced vascular endothelial cells and to investigate its mechanism.
AngII(10(-6)mol/L) was added to human vascular endothelial cells (HUVECs) culture media alone or with various concentration of Tongxinluo drug containing plasma (5%,10%, and 20%) added 30 minutes before AngII. Cell viability was evaluated after 24-hour incubation with AngII in a dose manner. TF, AngII type 1 receptor (AT(1)) mRNA, NO synthase (NOS) and NO were observed after 24-hour incubation with AngII. In addition, NOS inhibitor nomega-nitro-larginine (L-NAME) was added 30 minutes before Tongxinluo and AngII. Cell viability, TF, AT(1)mRNA, the level of NOS and NO were evaluated after 24-hour incubation with Tongxinluo and AngII.
Tongxinluo significantly improved AngII induced endothelial cell viability and the effect was the most obvious at 10%. Tongxinluo (10%) decreased the TF and AT(1) mRNA while increased the NOS and NO levels. L-NAME obviously inhibited the effects of Tongxinluo on cell viability, TF, AT(1) mRNA, and NOS and NO levels.
Up-regulating NOS-NO signaling may be the mechanism of Tongxinluo on cell viability and TF in AngII induced vacular endothelial cells.
探讨通心络对血管紧张素II（AngII）诱导的血管内皮细胞活力及组织因子（TF）的影响，并研究其作用机制。
将AngII（10(-6)mol/L）单独加入人血管内皮细胞（HUVECs）培养基中，或在加入AngII前30分钟加入不同浓度（5%、10%和20%）含通心络药物的血浆。与AngII孵育24小时后，以剂量方式评估细胞活力。与AngII孵育24小时后，观察TF、血管紧张素II 1型受体（AT(1)）mRNA、一氧化氮合酶（NOS）和一氧化氮（NO）。此外，在加入通心络和AngII前30分钟加入NOS抑制剂ω-硝基-L-精氨酸（L-NAME）。与通心络和AngII孵育24小时后，评估细胞活力、TF、AT(1)mRNA、NOS水平和NO水平。
通心络显著改善了AngII诱导的内皮细胞活力，10%浓度时效果最明显。通心络（10%）降低了TF和AT(1) mRNA水平，同时提高了NOS和NO水平。L-NAME明显抑制了通心络对细胞活力、TF、AT(1) mRNA、NOS水平和NO水平的影响。
上调NOS-NO信号通路可能是通心络改善AngII诱导的血管内皮细胞活力及TF水平的作用机制。
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