纤维蛋白对人血管内皮细胞组织因子表达的诱导作用
Fibrin induction of tissue factor expression in human vascular endothelial cells.
作者信息
Contrino J, Goralnick S, Qi J, Hair G, Rickles F R, Kreutzer D L
机构信息
Department of Medicine, University of Connecticut Health Center, Farmington 06030, USA.
出版信息
Circulation. 1997 Jul 15;96(2):605-13.
BACKGROUND
For the present study, we hypothesized that fibrin is an inducer of tissue factor (TF) expression in vascular endothelial cells in vitro and in vivo.
METHODS AND RESULTS
To test the in vitro aspect of this hypothesis, human umbilical vein endothelial cells (HUVECs) were cocultured with physiologically relevant concentrations of fibrin (0.03 to 1.0 mg fibrin/mL) for various times (0.5 to 24 hours), and TF expression was compared with that in unstimulated HUVECs (media control). Results demonstrated that fibrin induced a time- and dose-dependent increase in TF antigen expression, functional TF procoagulant activity, and TF mRNA in HUVECs.
CONCLUSIONS
These studies demonstrate that fibrin can directly regulate TF expression in HUVECs in vitro.
背景
在本研究中，我们假设纤维蛋白是体外和体内血管内皮细胞中组织因子（TF）表达的诱导剂。
方法与结果
为了验证这一假设的体外情况，将人脐静脉内皮细胞（HUVECs）与生理相关浓度的纤维蛋白（0.03至1.0毫克纤维蛋白/毫升）共培养不同时间（0.5至24小时），并将TF表达与未刺激的HUVECs（培养基对照）进行比较。结果表明，纤维蛋白可诱导HUVECs中TF抗原表达、功能性TF促凝活性和TF mRNA呈时间和剂量依赖性增加。
结论
这些研究表明，纤维蛋白可在体外直接调节HUVECs中的TF表达。
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