Fujibayashi Yuka, Fujimori Yoshihiro, Kasumoto Ikuyo, Kai Shunro, Hara Hiroshi, Okamura Haruki, Tsutsui Hiroko, Ogawa Hiroyasu, Nakanishi Kenji
Laboratory of Cell Transplantation, Institute for Advanced Medical Sciences, Hyogo College of Medicine, Hyogo 663-8501, Japan.
Int J Mol Med. 2007 Aug;20(2):241-5.
Natural killer T (NKT) cells, exhibiting both T-cell and NK-cell markers, are known to regulate immune responses by secreting T-helper (Th) 1 and Th2 cytokines. We analyzed NKT cells in cord blood (CB) for phenotypical and functional characteristics and regulatory mechanisms that control Th1 and Th2 determination. Human CB V alpha 24+V beta 11+ NKT cells were predominantly the CD4+ single positive (SP) phenotype (approximately 96%), in contrast to adult peripheral blood V alpha 24+V beta 11+ NKT cells which are composed of a dominant population of the CD4-CD8-double negative (DN) phenotype and a minor population of the CD4+ SP phenotype. The CB CD4+ V alpha 24+ NKT cells, following stimulation with the primary culture, gained the capacity to secrete interferon (IFN)-gamma, a Th1 cytokine, and interleukin (IL)-13, a Th2 cytokine. The combination of IL-18 and IL-12 induced IFN-gamma production in CB CD4+ V alpha 24+ NKT cells, while IL-18 in combination with IL-2 induced IL-13 production in these cells. Thus, IL-18 regulates the determination of the Th1 or Th2 immune response by human CD4+ V alpha 24+ NKT cells through different cytokine combinations.
自然杀伤T（NKT）细胞同时表达T细胞和NK细胞标志物，已知其通过分泌辅助性T（Th）1和Th2细胞因子来调节免疫反应。我们分析了脐血（CB）中的NKT细胞，以了解其表型和功能特征以及控制Th1和Th2分化的调节机制。与成人外周血Vα24 + Vβ11 + NKT细胞不同，人类CB Vα24 + Vβ11 + NKT细胞主要为CD4 +单阳性（SP）表型（约96%），成人外周血Vα24 + Vβ11 + NKT细胞由占主导地位的CD4 - CD8 -双阴性（DN）表型群体和少量CD4 + SP表型群体组成。CB CD4 + Vα24 + NKT细胞在原代培养刺激后，获得了分泌Th1细胞因子干扰素（IFN）-γ和Th2细胞因子白细胞介素（IL）-13的能力。IL-18和IL-12的组合诱导CB CD4 + Vα24 + NKT细胞产生IFN-γ，而IL-18与IL-2组合则诱导这些细胞产生IL-13。因此，IL-18通过不同的细胞因子组合调节人类CD4 + Vα24 + NKT细胞的Th1或Th2免疫反应分化。
