通过国家动物卫生监测系统的2000年猪研究获取的猪粪便样本进行大肠杆菌O157:H7检测。
Testing of swine feces obtained through the National Animal Health Monitoring System's Swine 2000 study for the presence of Escherichia coli O157:H7.
作者信息
Feder Ingrid, Gray Jeffrey T, Pearce Rachel A, Fratamico Pina M, Bush Eric, Porto-Fett Anna, Wallace F Morgan, Fedorka-Cray Paula J, Luchansky John B
机构信息
U.S. Department of Agriculture, Agricultural Research Service, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA.
出版信息
J Food Prot. 2007 Jun;70(6):1489-92. doi: 10.4315/0362-028x-70.6.1489.
Fecal samples collected from healthy pigs from 13 of the top 17 swine-producing states were tested for Escherichia coli O157:H7 as part of the National Animal Health Monitoring System Swine 2000 study. Serogroup O157 strains were isolated from 106 of 2,526 fecal samples. None of the isolates were positive by PCR for the fliCh7 (H7 flagellin) gene or for the hly933 (hemolysin) gene; however, one isolate was positive for the stxl gene (Shiga toxin 1), an additional four isolates were positive for the stx2 gene (Shiga toxin 2), and three isolates possessed the eae gene (intimin).
作为国家动物卫生监测系统2000年猪类研究的一部分，从17个生猪养殖大省中排名前13的省份的健康猪采集粪便样本，检测其中的大肠杆菌O157:H7。在2526份粪便样本中，有106份分离出了O157血清型菌株。通过聚合酶链反应（PCR）检测，这些分离株中没有一个fliCh7（H7鞭毛蛋白）基因或hly933（溶血素）基因呈阳性；然而，有一个分离株stx1基因（志贺毒素1）呈阳性，另外四个分离株stx2基因（志贺毒素2）呈阳性，还有三个分离株携带eae基因（紧密素）。
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