蛛网膜下腔出血后兔基底动脉中白细胞介素-8基因的表达变化
Expression change of interleukin-8 gene in rabbit basilar artery after subarachnoid hemorrhage.
作者信息
Wang Yong, Zhong Ming, Tan Xian-Xi, Yang Yun-Jun, Chen Wei-Jian, Liu Wei, Zheng Kuang
机构信息
Department of Neurosurgery, the First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China.
出版信息
Neurosci Bull. 2007 May;23(3):151-5. doi: 10.1007/s12264-007-0022-1.
OBJECTIVE
To study the expression change of interleukin-8 (IL-8) gene in the basilar artery of rabbit and the effect of IL-8 on the development of cerebral vasospasm induced by experimental subarachnoid hemorrhage (SAH).
METHODS
Thirty five healthy Japanese White Rabbits were randomly divided into saline-control group and experimental group. The experimental group was subdivided into four groups, representing day 1, 4, 7 and 14 after the first blood injection of SAH. The delayed cerebral vasospasm (DCVS) model was established by double injection of autologous blood into the cisterna magna. The expression change of cytokine IL-8 mRNA in the basilar artery was analyzed by RTPCR.
RESULTS
The expression of IL-8 gene increased on day 4-7 after the first blood injection of SAH compared with control (P< 0.001), and decreased to normal on day 14. The expression of IL-8 gene in the SAH groups were positively correlated with the degree of basilar artery stenosis (r = 0.642, P< 0.01).
CONCLUSION
The expression level of IL-8 gene in basilar arteries was intimately associated with the degree of cerebral vasospasm, suggesting that IL-8 may play an important role in the DCVS after SAH as an immunological inflammatory factor.
目的
研究白细胞介素-8（IL-8）基因在兔基底动脉中的表达变化以及IL-8对实验性蛛网膜下腔出血（SAH）所致脑血管痉挛发展的影响。
方法
将35只健康日本大白兔随机分为生理盐水对照组和实验组。实验组再分为四组，分别代表SAH首次注血后第1天、第4天、第7天和第14天。通过向枕大池两次注入自体血建立迟发性脑血管痉挛（DCVS）模型。采用逆转录聚合酶链反应（RT-PCR）分析基底动脉中细胞因子IL-8 mRNA的表达变化。
结果
与对照组相比，SAH首次注血后第4至7天IL-8基因表达增加（P<0.001），第14天降至正常。SAH各组中IL-8基因表达与基底动脉狭窄程度呈正相关（r = 0.642，P<0.01）。
结论
基底动脉中IL-8基因表达水平与脑血管痉挛程度密切相关，提示IL-8作为一种免疫炎症因子可能在SAH后的DCVS中起重要作用。
Zotero 插件