Elkind-Hirsch K E, Valdes C T, Rogers D G
Department of Medicine, Methodist Hospital, Houston, Texas.
Horm Metab Res. 1991 Oct;23(10):481-5. doi: 10.1055/s-2007-1003734.
We recently found circulating corticosterone (CS) levels to be significantly lower in diabetic female rats as compared with proestrous control animals. This reduction in CS was correlated with the hypoestrogenic state of the diabetic female. It was the purpose of this study to evaluate basal and corticotropin releasing hormone (CRH)-stimulated CS secretion in ovariectomized (OVX) control (C) and streptozotocin-induced diabetic (D) rats given blank, 5 mcg and 20 mcg estradiol (E2) implants to determine if adrenal CS secretion in the diabetic is normalized by E2 treatment. After 3 weeks of diabetes, pituitary-adrenal function was assessed in rats from each group with a CRH stimulation test. The remaining rats were sacrificed for determination of CS, E2, testosterone and fructosamine in serum. Suppressed CS secretion in OVX female diabetic rats was partially restored with E2 therapy. Basal CS levels were significantly higher in 20 mcg E2 treated C and D rats compared with OVX rats. However, C rats had significantly higher basal CS compared with D rats in similarly E2 treated groups. The CS response to CRH stimulation was not different between OVX female diabetic and control rats. Estrogen enhanced the CS response to CRH stimulation in control animals but not in diabetic animals suggesting altered estrogen action at the pituitary level in diabetic animals.
我们最近发现，与处于动情前期的对照动物相比，糖尿病雌性大鼠体内循环皮质酮（CS）水平显著降低。CS的这种降低与糖尿病雌性大鼠的雌激素缺乏状态相关。本研究的目的是评估给予空白、5微克和20微克雌二醇（E2）植入物的去卵巢（OVX）对照（C）大鼠和链脲佐菌素诱导的糖尿病（D）大鼠的基础及促肾上腺皮质激素释放激素（CRH）刺激后的CS分泌情况，以确定糖尿病大鼠的肾上腺CS分泌是否通过E2治疗恢复正常。糖尿病3周后，通过CRH刺激试验评估每组大鼠的垂体-肾上腺功能。其余大鼠处死后测定血清中的CS、E2、睾酮和果糖胺。E2治疗可部分恢复OVX雌性糖尿病大鼠受抑制的CS分泌。与OVX大鼠相比，接受20微克E2治疗的C组和D组大鼠的基础CS水平显著更高。然而，在同样接受E2治疗的组中，C组大鼠的基础CS水平显著高于D组大鼠。OVX雌性糖尿病大鼠和对照大鼠对CRH刺激的CS反应没有差异。雌激素增强了对照动物对CRH刺激的CS反应，但在糖尿病动物中没有，这表明糖尿病动物垂体水平的雌激素作用发生了改变。
