Igarashi Tsutomu, Miyake Koichi, Hayakawa Jun, Kawabata Ken, Ishizaki Masamichi, Takahashi Hiroshi, Shimada Takashi
Department of Biochemistry, Nippon Medical School, Tokyo, Japan.
Curr Eye Res. 2007 Jun;32(6):543-53. doi: 10.1080/02713680701389333.
We studied the contribution made by circulating bone marrow (BM)-derived cells to the newborn and mature retinas of BM-transplanted mice.
Newborn and adult C57BL/6J mice were administered a lethal dose of total-body irradiation, after which pathologic changes to the retinas were periodically assessed. In addition, mice received BM cells from 8-week-old green fluorescent protein (GFP) transgenic mice, and the subsequent differentiation of GFP+ cells was studied.
Within 5 hr after irradiation of newborn mice, retinal cells began to die due to apoptosis. By contrast, irradiation of adult mice elicited no histologic changes in the retina. BM cells generally did not differentiate in adult mice, but numerous GFP+ BM cells were integrated into the retinal tissue of newborn mice, where they expressed various cell type-specific markers. Finally, examination of whole retina mounts showed that GFP+ cells also contributed to retinal vascularization.
Our findings underscore the importance of careful evaluation of the biological effects of irradiation in models making use of BM transplantation.
我们研究了循环骨髓（BM）来源的细胞对接受骨髓移植小鼠的新生视网膜和成熟视网膜的贡献。
对新生和成年C57BL/6J小鼠进行致死剂量的全身照射，之后定期评估视网膜的病理变化。此外，小鼠接受来自8周龄绿色荧光蛋白（GFP）转基因小鼠的骨髓细胞，并研究随后GFP+细胞的分化情况。
新生小鼠照射后5小时内，视网膜细胞开始因凋亡而死亡。相比之下，成年小鼠照射后视网膜未出现组织学变化。骨髓细胞在成年小鼠中一般不分化，但大量GFP+骨髓细胞整合到新生小鼠的视网膜组织中，在那里它们表达各种细胞类型特异性标志物。最后，对整个视网膜标本的检查表明，GFP+细胞也参与了视网膜血管形成。
我们的研究结果强调了在利用骨髓移植的模型中仔细评估照射生物学效应的重要性。
