Hamidi Mehrdad, Zarei Najmeh, Zarrin Abdolhossein, Mohammadi-Samani Soleiman
Department of Pharmaceutics, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Drug Deliv. 2007 Jul;14(5):295-300. doi: 10.1080/10717540701203000.
Erythrocytes as the most readily available and abundant cells within the body have been studied extensively for their potential application as drug delivery carries. In this study, human erythrocytes were loaded by bovine serum albumin (BSA) as a model antigen/protein using hypotonic preswelling method for targeted delivery of this antigen-to antigen-presenting cells. The average loaded amount, efficiency of entrapment, and cell recovery upon loading procedure were 1979.25 +/- 9.4 microg, 30.06 +/- 0.20%, and 87.53 +/- 0.66%, respectively. The total BSA recovery upon loading procedure was 97.20 +/- 4.90%. The apparent mechanism of entrapment was simple concentration-based gradient in/out the cells with some minor limiting factors against protein entry into the cells. We have shown that the intra- and intersubject variations of the method were interestingly low (i.e., less than 5% in all cases).
红细胞作为体内最容易获得且数量丰富的细胞，因其作为药物递送载体的潜在应用而受到广泛研究。在本研究中，使用低渗预膨胀方法，以牛血清白蛋白（BSA）作为模型抗原/蛋白质加载人红细胞，用于将该抗原靶向递送至抗原呈递细胞。加载过程中的平均加载量、包封效率和细胞回收率分别为1979.25±9.4微克、30.06±0.20%和87.53±0.66%。加载过程中BSA的总回收率为97.20±4.90%。明显的包封机制是基于细胞内外简单的浓度梯度，存在一些限制蛋白质进入细胞的次要因素。我们已经表明，该方法在个体内和个体间的差异低得出奇（即所有情况下均小于5%）。
