Yulda-Hanso-Tang attenuates the endotoxin-induced inflammatory cytokine production in peripheral blood mononuclear cells.

Yulda-Hanso-Tang (YHT) has been used as a formula for the cerebral infarction (CI) patients. This study investigated the anti-inflammatory effects and possible mechanism of YHT on lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells. The amount of interleukin-1beta, IL-4, IL-6, IL-8, and tumor necrosis factor-alpha was increased in the LPS-treated cells. But, increased cytokine was inhibited by YHT except IL-8. The study showed that YHT inhibited the IL-1beta mRNA expression. YHT also inhibited LPS-induced activation of NF-kappaB and degradation of IkappaBalpha. These results suggest that YHT is an inhibitor of NF-kappaB, which might explain its beneficial effect in the treatment of inflammatory diseases.