Yoon Myonggeun, Park Sung Yong, Shin Jungwook, Kwak Jungwon, Park Junghun, Shin Dongho, Park Soah, Lee Se Byeong, Lee Doo Hyun, Shin Kyung Hwan, Pyo Hong Ryull, Kim Joo-Young, Cho Kwan Ho
Radiation Oncology, Proton Therapy Center, National Cancer Center, Ilsandong-gu, Goyang, Korea.
Int J Radiat Biol. 2007 Aug;83(8):561-6. doi: 10.1080/09553000701383178.
Dose uniformity in cell culture vessels such as Petri dishes and anoxic irradiation chambers is very important in radiobiological work as dose uniformity affects cell survival probabilities. In this study, we investigated X-ray dose inhomogeneity, caused by scattering, in typical culture vessels.
Three different cubic cell culture vessels, with side lengths of 10 cm, 15 cm and 20 cm, were designed and irradiated by X-rays of 6 MV and 15 MV at a source-to-surface distance (SSD) of 100 cm using a Varian 2,100CD linear accelerator.
The relative X-ray dose distribution in a cell culture vessel depended strongly on whether the vessel had a lid. The percentage of the cell culture surface with the dose differing by more than 10% from the mean value of the dose was 43.4% in lidless vessels and 9.7% in lidded vessels.
In radiobiological work, X-ray dose inhomogeneity within a cell culture vessel is not negligible and the placement of cells in the vessel should be carefully considered.
在放射生物学研究中，细胞培养容器（如培养皿和缺氧照射室）内的剂量均匀性非常重要，因为剂量均匀性会影响细胞存活概率。在本研究中，我们调查了典型培养容器中由散射引起的X射线剂量不均匀性。
设计了三种边长分别为10 cm、15 cm和20 cm的不同立方体细胞培养容器，并使用Varian 2100CD直线加速器，在源皮距（SSD）为100 cm的条件下，用6 MV和15 MV的X射线对其进行照射。
细胞培养容器内的相对X射线剂量分布很大程度上取决于容器是否有盖子。在无盖容器中，剂量与剂量平均值相差超过10%的细胞培养表面百分比为43.4%，在有盖容器中为9.7%。
在放射生物学研究中，细胞培养容器内的X射线剂量不均匀性不可忽略，应仔细考虑细胞在容器中的放置位置。
