抗LipL32单克隆抗体在钩端螺旋体病免疫诊断试验中的应用潜力评估。

Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests.

作者信息

Coutinho M L, Vasconcellos F A, Fernandes C P H, Seyffert N, Seixas F K, Ko A I, Dellagostin O A, Aleixo J A G

机构信息

Center for Biotechnology, Federal University of Pelotas, Rio Grande do Sul, Brazil.

出版信息

J Immunoassay Immunochem. 2007;28(3):279-88. doi: 10.1080/15321810701454870.

DOI:10.1080/15321810701454870

PMID:17613673

Abstract

LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies (MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG(2b) isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5x10(7) M(-1) and 6x10(6) M(-1). These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection.

摘要

LipL32是致病性钩端螺旋体膜中的主要脂蛋白。在本研究中，我们报告了抗重组LipL32（rLipL32）单克隆抗体（MAb）的制备及其作为钩端螺旋体病诊断测试试剂的潜力评估。这些单克隆抗体均为IgG(2b)亚型，且仅与致病性血清型中的天然LipL32发生特异性反应。单克隆抗体在rLipL32分子的同一区域发生反应，其亲和常数在5×10(7) M(-1)至6×10(6) M(-1)之间。这些结果表明，虽然这些单克隆抗体不能一起使用，但它们非常适合基于LipL32检测的钩端螺旋体病诊断测试。