Rasjad C, Yamashita K, Datu A R, Yasuda M
Department of Anatomy, Hiroshima University School of Medicine, Japan.
Hiroshima J Med Sci. 1991 Sep;40(3):93-9.
The present study investigated the pattern of limb malformations induced in mice by methoxyacetic acid (MAA), one of di(2-methoxyethyl) phthalate (DMEP) metabolites. Pregnant Jcl:ICR mice were given orally at gestational day (gd) 10.5, 11.0, or 11.5 (vaginal plug = gd 0) a single dose of MAA 10 mmol/kg of body weight. Fetuses were examined at gd 15.5 for external and skeletal malformations. Limb defects were maximum in frequency and severity after administration at gd 11.5. Forelimbs had greater susceptibility than hindlimbs. Treatment at gd 10.5 produced cutaneous or osseous syndactyly between digits I and II, II and III, and ectrodactyly of digit II or digit I. Intercalary defects in the forelimbs were also detected. No hindlimb malformations were induced. Treatment at gd 11.0 induced ectrodactyly of digit II and digit I in the forelimbs, as well as osseous syndactyly or intercalary defects of the metacarpals or phalanges, and the frequency became increased. Hindlimb malformations were also detected in a small number with syndactyly between digits I and II or ectrodactyly in digit I. Treatment at gd 11.5 induced ectrodactyly in the forelimbs. Half of the forelimbs showed ectrodactyly with four missing digits (digits I, II, III, and V) and the remaining limbs showed ectrodactyly with a similar frequency with one, two, or three missing digits, either in the pre- or postaxial area. In the hindlimbs ectrodactyly with one, two, or three missing digits was the most common malformation observed. Syndactyly between digits I and II was also induced in a small percentage.
本研究调查了邻苯二甲酸二（2-甲氧基乙基）酯（DMEP）代谢物之一甲氧基乙酸（MAA）诱导小鼠肢体畸形的模式。在妊娠第（gd）10.5、11.0或11.5天（阴栓=gd 0），给怀孕的Jcl:ICR小鼠口服单剂量10 mmol/kg体重的MAA。在gd 15.5检查胎儿的外部和骨骼畸形。在gd 11.5给药后，肢体缺陷的频率和严重程度最高。前肢比后肢更易受影响。在gd 10.5进行处理会导致第I和II指、II和III指之间出现皮肤或骨性并指，以及第II指或第I指的缺指畸形。在前肢也检测到了间插性缺陷。未诱导出后肢畸形。在gd 11.0进行处理会导致前肢第II指和第I指出现缺指畸形，以及掌骨或指骨的骨性并指或间插性缺陷，且频率增加。在少数后肢中也检测到畸形，表现为第I和II指之间并指或第I指缺指畸形。在gd 11.5进行处理会导致前肢出现缺指畸形。一半的前肢表现为缺指畸形，缺失四个手指（第I、II、III和V指），其余肢体出现缺指畸形的频率相似，缺失一个、两个或三个手指，位于轴前或轴后区域。在后肢中，缺失一个、两个或三个手指的缺指畸形是最常见的畸形。第I和II指之间的并指畸形也在小部分中被诱导出来。
