鉴定参与骨重塑及抑制病理性钙化的骨桥蛋白磷酸化位点。
Identification of osteopontin phosphorylation sites involved in bone remodeling and inhibition of pathological calcification.
作者信息
Saad Fawzy A, Salih Erdjan, Glimcher Melvin J
机构信息
Laboratory for Study of Skeletal Disorders and Rehabilitation, Department of Orthopaedic Surgery, Harvard Medical School, Children's Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA.
出版信息
J Cell Biochem. 2008 Feb 15;103(3):852-6. doi: 10.1002/jcb.21453.
Abstract
Osteopontin is a noncollagenous, phosphorylated extracellular glycoprotein, expressed in mineralized and nonmineralized tissues, organs and body fluids. The protein contains an RGD tripeptide cell-binding motif, and is subjected to a variety of posttranslational modifications that play important roles in its multiple biological functions, such as bone remodeling and inhibition of pathological calcification. In this study, we have expressed bovine osteopontin in a prokaryotic system and identified the seven amino acid residues phosphorylated in vitro by CKII.
摘要
骨桥蛋白是一种非胶原蛋白的磷酸化细胞外糖蛋白，在矿化和非矿化组织、器官及体液中表达。该蛋白含有一个RGD三肽细胞结合基序，并经历多种翻译后修饰，这些修饰在其多种生物学功能中发挥重要作用，如骨重塑和抑制病理性钙化。在本研究中，我们在原核系统中表达了牛骨桥蛋白，并鉴定了体外被CKII磷酸化的七个氨基酸残基。
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2
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Matrix Biol. 2007 Jan;26(1):42-53. doi: 10.1016/j.matbio.2006.09.002. Epub 2006 Sep 15.
3
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