Wu Qiang-Sheng, Zou Ying-Ning, Xia Ren-Xue
College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China.
Ying Yong Sheng Tai Xue Bao. 2007 Apr;18(4):825-30.
In a pot experiment, this paper studied the effects of arbuscular mycorrhizal (AM) fungus Glomus versiforme (Karsten) Berch inoculation on the reactive oxygen metabolism of Citrus tangerine Hort. ex Tanaka leaves under water stress. The results showed that water stress decreased the colonization of G. versiforme on C. tangerine roots significantly, with a decrement of 33%. Under normal water supply and water stress, G. versiforme inoculation increased the leaf P content by 45% and 27%, and decreased the leaf malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents by 25% and 21%, and 16% and 16%, respectively, compared with the control. Inoculation with G. versiforme enhanced the activities of leaf superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX), and increased the contents of leaf soluble protein, ascorbate (ASC) and total ascorbate (TASC) notably, regardless soil moisture condition. Under water stress, G. versiforme inoculation decreased the leaf superoxide anion radical (O2-*) content by 31%, compared with that under normal water supply. It was suggested that the drought resistance of C. tangerine leaves was enhanced after G. versiforme inoculation.
通过盆栽试验，研究了接种丛枝菌根（AM）真菌地表球囊霉（Glomus versiforme (Karsten) Berch）对水分胁迫下柑橘（Citrus tangerine Hort. ex Tanaka）叶片活性氧代谢的影响。结果表明，水分胁迫显著降低了地表球囊霉在柑橘根际的定殖率，降幅为33%。与对照相比，在正常供水和水分胁迫条件下，接种地表球囊霉分别使叶片磷含量提高了45%和27%，使叶片丙二醛（MDA）和过氧化氢（H2O2）含量分别降低了25%和21%、16%和16%。无论土壤水分条件如何，接种地表球囊霉均能增强叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）的活性，并显著提高叶片可溶性蛋白、抗坏血酸（ASC）和总抗坏血酸（TASC）的含量。水分胁迫下，接种地表球囊霉的柑橘叶片超氧阴离子自由基（O2-*）含量比正常供水条件下降低了31%。由此表明，接种地表球囊霉后增强了柑橘叶片的抗旱性。
