Wang Zeng-jun, Zhang Wei, Wu Hong-fei, Sui Yuan-geng
Department of Urology, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210029, China.
Zhonghua Nan Ke Xue. 2007 Jun;13(6):498-501.
To further study gene expression and characterization of voltage-dependent anion channels (VDACs) on human spermatozoa.
VDACs were cloned by PCR from the testis cDNA library. Recombinant human sperm VDACs were produced in E. coli system by molecular cloning technology. Sperm membrane protein was extracted by 1% Triton X-100 and separated by chloroform/methanol.
The gene expression of VDACs was found in the human testis cDNA library and VDAC protein was detected located on the sperm membrane by alpha-helix.
VDAC proteins, abundant on the human sperm membrane and responsible for anion transportation, play an important role in sperm signaling transduction and fertility.
进一步研究人精子上电压依赖性阴离子通道（VDACs）的基因表达及特性。
通过PCR从睾丸cDNA文库中克隆VDACs。利用分子克隆技术在大肠杆菌系统中制备重组人精子VDACs。用1% Triton X-100提取精子膜蛋白，并用氯仿/甲醇进行分离。
在人睾丸cDNA文库中发现了VDACs的基因表达，通过α-螺旋检测到VDAC蛋白位于精子膜上。
VDAC蛋白在人精子膜上含量丰富，负责阴离子转运，在精子信号转导和生育能力方面发挥重要作用。
