Rahmani Z, Maunoury C, Siddiqui A
Department of Microbiology, University of Colorado Health Sciences Center, Denver, USA.
Eur J Hum Genet. 1998 Jul-Aug;6(4):337-40. doi: 10.1038/sj.ejhg.5200198.
The voltage-dependent anion channel of the mitochondrial outer membrane (VDAC) is a small, abundant pore-forming protein found in the outer membranes of all eukaryotic mitochondria. The VDAC protein is believed to control the movement of adenine nucleotides through the outer membrane and to be the mitochondrial binding site for hexokinase and glycerol kinase. Two human VDAC cDNAs (HVDAC1 and HVDAC2) have been previously isolated and mapped on chromosome X and 21, respectively. Here, we report the isolation of a novel third human VDAC cDNA, corresponding to the mouse MVDAC3 gene. The expression of this gene in various tissues and its chromosomal localization by polymerase chain reaction (PCR) using a human/rodent somatic cell mapping panel and by fluorescence in situ hybridization is also presented.
线粒体外膜的电压依赖性阴离子通道（VDAC）是一种存在于所有真核生物线粒体的外膜中的小型、丰富的成孔蛋白。VDAC蛋白被认为可控制腺嘌呤核苷酸通过外膜的移动，并且是己糖激酶和甘油激酶的线粒体结合位点。此前已分离出两个人类VDAC cDNA（HVDAC1和HVDAC2），它们分别定位于X染色体和21号染色体上。在此，我们报告分离出一种新的第三个人类VDAC cDNA，它对应于小鼠MVDAC3基因。还介绍了该基因在各种组织中的表达情况，以及通过使用人/啮齿动物体细胞定位板进行聚合酶链反应（PCR）和荧光原位杂交对其进行的染色体定位。
