Giraldez-Perez Rosa M, Gaytan Susana P, Ruano Diego, Torres Blas, Pasaro Rosario
Department of Fisiología y Zoología, Facultad de Biología, Universidad de Sevilla, Sevilla 41012, Spain.
J Chem Neuroanat. 2008 Jan;35(1):12-32. doi: 10.1016/j.jchemneu.2007.05.007. Epub 2007 May 31.
The nitrergic system has been inferred from cells positive to nicotinamide adenine dinucleotide phosphate diaphorase (NADPHd) histochemistry and/or to the neuronal isoform of nitric oxide synthase (nNOS) immunohistochemistry in different species of vertebrates. The aim of the present work was to systematically study the distribution of cell producing nitric oxide in the goldfish (Carassius auratus) brain. To reach this goal, we firstly studied co-localization for NADPHd and nNOS techniques and demonstrated an extensive double labeling. Then, we studied the distribution through the brain by the two separate methods and found labeled cells widely distributed in brain and spinal cord. In the telencephalon, such cells were in both dorsal and ventral areas. In the diencephalon, the cells were found in some nuclei of the preoptic area and hypothalamus, habenula, pretectum, and dorsal and ventral thalamic regions. In the midbrain, cells were observed in the optic tectum, torus longitudinalis, and tegmental nuclei. In the rhombencephalon, cells were found in the cerebellum, the reticular formation, the locus coeruleus, the raphe nuclei, and the nuclei of the cranial nerves. Labeled cells were also observed in the gray area of the spinal cord. Cognizing that a direct comparison of the present results with those reported in other vertebrates is not clear-cut because of homologies; we conclude that the nitrergic system is roughly similar from fish to mammals.
在不同种类的脊椎动物中，已通过对烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPHd）组织化学呈阳性和/或对一氧化氮合酶（nNOS）神经元亚型免疫组织化学呈阳性的细胞推断出一氧化氮能系统。本研究的目的是系统地研究金鱼（Carassius auratus）脑中产生一氧化氮的细胞的分布。为了实现这一目标，我们首先研究了NADPHd和nNOS技术的共定位，并证明了广泛的双重标记。然后，我们通过两种单独的方法研究了整个大脑中的分布，发现标记细胞广泛分布于脑和脊髓中。在端脑中，此类细胞分布于背侧和腹侧区域。在间脑中，细胞见于视前区和下丘脑、缰核、顶盖前区以及背侧和腹侧丘脑区域的一些核团中。在中脑中，细胞见于视顶盖、纵纹隆起和被盖核。在菱脑中，细胞见于小脑、网状结构、蓝斑、中缝核和脑神经核。在脊髓灰质区域也观察到了标记细胞。鉴于由于同源性，目前的结果与其他脊椎动物报道的结果进行直接比较并不明确；我们得出结论，从鱼类到哺乳动物，一氧化氮能系统大致相似。
