Ito H
J Clin Microbiol. 1976 Feb;3(2):91-5. doi: 10.1128/jcm.3.2.91-95.1976.
The MN strain of Sendai virus formed distinct plaques in monolayers of PS-Y15 cells, an established porcine kidney cell line. The plaque-forming ability was neutralized by specific antibody to the virus. A linear relationship was found between the concentration of virus and the number of plaques. The sensitivity of this assay was about equal to that of the in ovo titration. When applied to the serum neutralization test, the end points obtained were comparable to those of the hemagglutination-inhibition and complement-fixation tests.
仙台病毒的MN株在已建立的猪肾细胞系PS-Y15细胞单层中形成明显的蚀斑。蚀斑形成能力被该病毒的特异性抗体中和。发现病毒浓度与蚀斑数量之间呈线性关系。该检测方法的灵敏度与鸡胚滴定法大致相同。应用于血清中和试验时，得到的终点结果与血凝抑制试验和补体结合试验的结果相当。
