Ryoo Sung Weon, Park Young Kil, Park Sue-Nie, Shim Young Soo, Liew Hyunjeong, Kang Seongman, Bai Gill-Han
Department of Microbiology, Korean Institute of Tuberculosis, Seoul, Republic of Korea.
J Microbiol. 2007 Jun;45(3):268-71.
In Korea, the Mycobacterium tuberculosis K-strain is the most prevalent clinical isolates and belongs to the Beijing family. In this study, we conducted comparative porteomics of expressed proteins of clinical isolates of the K-strain with H37Rv, H37Ra as well as the vaccine strain of Mycobacterium bovis BCG following phagocytosis by the human monocytic cell line U-937. Proteins were analyzed by 2-D PAGE and MALDITOF-MS. Two proteins, Mb1363 (probable glycogen phosphorylase GlgP) and MT2656 (Haloalkane dehalogenase LinB) were most abundant after phagocytosis of M. tuberculosis K-strain. This approach provides a method to determine specific proteins that may have critical roles in tuberculosis pathogenesis.
在韩国，结核分枝杆菌K株是最常见的临床分离株，属于北京家族。在本研究中，我们对人单核细胞系U-937吞噬结核分枝杆菌K株、H37Rv、H37Ra以及牛分枝杆菌卡介苗疫苗株后的临床分离株表达蛋白进行了比较蛋白质组学研究。通过二维聚丙烯酰胺凝胶电泳和基质辅助激光解吸电离飞行时间质谱对蛋白质进行分析。结核分枝杆菌K株被吞噬后，两种蛋白Mb1363（可能的糖原磷酸化酶GlgP）和MT2656（卤代烷脱卤酶LinB）最为丰富。该方法提供了一种确定可能在结核病发病机制中起关键作用的特定蛋白质的方法。
