抗人DR5单克隆抗体mDRA-6诱导白血病细胞凋亡
[Leukemic cell apoptosis induced by anti-human DR5 monoclonal antibody mDRA-6].
作者信息
Li Shu-lian, Ma Yuan-fang, Liu Guang-chao, Zhang Jun, Bai Hui-ling, Liu Ying-jie
机构信息
Institute of Immunology, Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng 475004, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Aug;23(8):754-6.
AIM
To investigate the apoptotic effect of anti-human DR5 (death receptor 5 of TRAIL) monoclonal antibody mDRA-6 on leukemic cells.
METHODS
The morphological changes of leukemic cells were observed by fluorescence microscope. The cytotoxic and apoptotic effects of mDRA-6 on Jurkat, HL-60 and K562 cells were detected by MTT analysis and flow cytometry with Annexin V-FITC/PI staining.
RESULTS
Chromatin condensation, budding and apoptotic bodies were observed in Jurkat and HL-60 cells treated by mDRA-6. Death and apoptosis of leukemic cells treated by mDRA-6 were increased, but the effect of mDRA-6 on K562 cells was not obvious.
CONCLUSION
Apoptosis of leukemic cells can be induced by anti-human DR5 monoclonal antibody mDRA-6. Different leukemic cell lines are of different sensitivity to mDRA-6.
目的
研究抗人DR5（肿瘤坏死因子相关凋亡诱导配体的死亡受体5）单克隆抗体mDRA-6对白血病细胞的凋亡作用。
方法
通过荧光显微镜观察白血病细胞的形态变化。采用MTT分析法及Annexin V-FITC/PI染色流式细胞术检测mDRA-6对Jurkat、HL-60和K562细胞的细胞毒性及凋亡作用。
结果
mDRA-6处理后的Jurkat和HL-60细胞可见染色质凝聚、出芽及凋亡小体。mDRA-6处理的白血病细胞死亡及凋亡增加，但mDRA-6对K562细胞的作用不明显。
结论
抗人DR5单克隆抗体mDRA-6可诱导白血病细胞凋亡。不同白血病细胞系对mDRA-6的敏感性不同。
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