藏羚羊α-珠蛋白基因编码区的克隆及同源性分析
[Cloning and homologous analysis of coding region of alpha-globin gene from Tibetan antelope].
作者信息
Yang Ying-zhong, Bai Zhen-zhong, Jin Guo-en, Ma Lan, Yun Hai-xia, Ge Ri-li
机构信息
Research Center for High Altitude Medical Sciences, Medical College of Qinghai University, Xining 810001, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 Jul;23(7):620-2.
AIM
To clone and analyze the encoding region of alpha-globin gene from Tibetan antelope.
METHODS
Total RNA was isolated from an adolescent Tibetan antelope liver, and Tibetan antelope alpha-globin gene was amplified by RT-PCR. The PCR product was cloned into pGEM-T vector and sequenced. Nucleotide sequences were compared with GenBank data by Blast method.
RESULTS
The encoding region of alpha-globin gene of Tibetan antelope was obtained and deposited in GenBank as accession number DQ650713. Compared with sheep alpha-chain, alterations in important regions could be noted: a132 Asn-->Ser, a134 Ser-->Gly; but 19 differences were detected when compared with that of human. Phylogenetic analysis showed that the encoding region of alpha-globin gene of Tibetan antelope was most likely close to that of sheep and goat.
CONCLUSION
The encoding region of gene Tibetan antelope alpha-globin gene is successfully cloned, which provides basic information for elucidating the possible role of hemoglobin in high altitude adaptation of Tibetan antelope.
目的
克隆并分析藏羚羊α-珠蛋白基因的编码区。
方法
从一只青春期藏羚羊肝脏中提取总RNA，通过逆转录聚合酶链反应（RT-PCR）扩增藏羚羊α-珠蛋白基因。将PCR产物克隆到pGEM-T载体中并测序。通过Blast方法将核苷酸序列与GenBank数据进行比较。
结果
获得了藏羚羊α-珠蛋白基因的编码区，并以登录号DQ650713存入GenBank。与绵羊α链相比，重要区域存在变化：a132天冬酰胺→丝氨酸，a134丝氨酸→甘氨酸；但与人类相比检测到19处差异。系统发育分析表明，藏羚羊α-珠蛋白基因的编码区最有可能与绵羊和山羊的编码区接近。
结论
成功克隆了藏羚羊α-珠蛋白基因的编码区，为阐明血红蛋白在藏羚羊高原适应中的可能作用提供了基础信息。
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