小于胎龄儿新生儿胎盘组织中皮质醇激活酶和皮质醇失活酶的基因表达同时降低。
Simultaneously reduced gene expression of cortisol-activating and cortisol-inactivating enzymes in placentas of small-for-gestational-age neonates.
作者信息
Struwe Ellen, Berzl Gabriele M, Schild Ralf L, Beckmann Matthias W, Dörr Helmuth G, Rascher Wolfgang, Dötsch Jörg
机构信息
Department of Pediatrics, University of Erlangen-Nürnberg, Nürnberg, Germany.
出版信息
Am J Obstet Gynecol. 2007 Jul;197(1):43.e1-6. doi: 10.1016/j.ajog.2007.02.012.
OBJECTIVE
The enzyme 11beta-hydroxysteroid dehydrogenase type 2 (11beta-HSD2) converts cortisol into cortisone. Reduced placental activity of 11beta-HSD2 in small-for-gestational-age (SGA) neonates results in fetal cortisol excess. In the present study, we examined the yet unknown gene expression of 11beta-HSD1, which primarily synthesizes cortisol in SGA placenta.
STUDY DESIGN
In placentas taken from 24 women with normal-weight newborns and 16 women with SGA neonates, expression of 11beta-HSD1 and 11beta-HSD2 messenger ribonucleic acid (mRNA) was determined using reverse transcription-polymerase chain reaction.
RESULTS
Placental mRNA expression of 11beta-HSD1 and 11beta-HSD2 was significantly reduced in the SGA group (P = .006 and P < .0001). Both enzymes showed a significant correlation to birthweight SD score and placental weight. Also, levels of both enzymes were significantly correlated.
CONCLUSION
In placental tissue of SGA neonates 11beta-HSD2 and 11beta-HSD1 gene expression is reduced. Adapted levels of 11beta-HSD1 might result in a counterregulatory mechanism limiting transplacental passage of elevated cortisol levels.
目的
2型11β-羟基类固醇脱氢酶（11β-HSD2）可将皮质醇转化为可的松。小于胎龄（SGA）新生儿胎盘11β-HSD2活性降低会导致胎儿皮质醇过量。在本研究中，我们检测了SGA胎盘中主要合成皮质醇的11β-HSD1的基因表达情况，此前该情况尚不清楚。
研究设计
从24例体重正常新生儿的母亲和16例SGA新生儿的母亲获取胎盘，采用逆转录-聚合酶链反应测定11β-HSD1和11β-HSD2信使核糖核酸（mRNA）的表达。
结果
SGA组胎盘11β-HSD1和11β-HSD2的mRNA表达显著降低（P = .006和P < .0001）。这两种酶均与出生体重标准差评分和胎盘重量显著相关。此外，这两种酶的水平也显著相关。
结论
SGA新生儿的胎盘组织中11β-HSD2和11β-HSD1基因表达降低。11β-HSD1水平的适应性变化可能导致一种反调节机制，限制升高的皮质醇水平经胎盘转运。
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