Petridou Anatoli, Tsalouhidou Sofia, Tsalis George, Schulz Thorsten, Michna Horst, Mougios Vassilis
Department of Physical Education and Sport Science, Aristotle University of Thessaloniki, 541 24 Thessaloniki, Greece.
Metabolism. 2007 Aug;56(8):1029-36. doi: 10.1016/j.metabol.2007.03.011.
The aim of the present study was to examine the effect of 8 weeks of voluntary wheel running on the gene expression, at the protein level, of 2 enzymes involved in lipogenesis (fatty acid synthase [FAS] and diacylglycerol acyl transferase 1), 2 proteins involved in lipolysis (hormone-sensitive lipase [HSL] and perilipin), and 3 transcription factors mediating the induction of genes involved in lipid metabolism (the alpha, gamma, and delta members of the peroxisome proliferator-activated receptor, or PPAR, family) in rat liver, gastrocnemius muscle, epididymal fat, and subcutaneous fat. Proteins were measured through Western blot analysis in the tissues of 11 trained and 14 untrained rats. The trained rats had lower FAS in the liver; higher FAS, HSL, and perilipin in epididymal fat; and higher HSL in subcutaneous fat. In addition, the trained rats had higher total protein concentrations in both fat depots. No significant differences in the liver, muscle, or adipose tissue PPAR contents were found between groups. However, the DNA binding activity of PPARgamma, measured through an enzyme immunoassay-based method, was higher in both fat depots of the trained rats. Our findings suggest that long-term wheel running had significant effects on the concentrations of proteins playing key roles in lipogenesis and lipolysis in rat liver and adipose tissue. These effects may be due to PPAR activation rather than induction, rendering the transcriptional regulation of target genes more economical and flexible. The activation of PPARgamma with exercise may mediate its beneficial effect on insulin sensitivity.
本研究的目的是检测8周的自愿轮转跑步对大鼠肝脏、腓肠肌、附睾脂肪和皮下脂肪中参与脂肪生成的2种酶（脂肪酸合酶[FAS]和二酰甘油酰基转移酶1）、参与脂肪分解的2种蛋白质（激素敏感性脂肪酶[HSL]和 perilipin）以及介导脂质代谢相关基因诱导的3种转录因子（过氧化物酶体增殖物激活受体[PPAR]家族的α、γ和δ成员）在蛋白质水平上的基因表达的影响。通过蛋白质印迹分析测定了11只训练大鼠和14只未训练大鼠组织中的蛋白质。训练大鼠肝脏中的FAS较低；附睾脂肪中的FAS、HSL和perilipin较高；皮下脂肪中的HSL较高。此外，训练大鼠的两个脂肪库中的总蛋白浓度均较高。两组之间在肝脏、肌肉或脂肪组织的PPAR含量上未发现显著差异。然而，通过基于酶免疫测定的方法测得，训练大鼠的两个脂肪库中PPARγ的DNA结合活性较高。我们的研究结果表明，长期轮转跑步对大鼠肝脏和脂肪组织中在脂肪生成和脂肪分解中起关键作用的蛋白质浓度有显著影响。这些影响可能是由于PPAR的激活而非诱导，使靶基因的转录调控更加经济和灵活。运动激活PPARγ可能介导其对胰岛素敏感性的有益作用。
