Minervino Antonio H H, Ragozo Alessandra M A, Monteiro Renata M, Ortolani Enrico L, Gennari Solange M
Faculdade de Medicina Veterinária e Zootecnia, Departamento de Clínica Médica, USP, São Paulo, SP, Brazil.
Res Vet Sci. 2008 Apr;84(2):254-6. doi: 10.1016/j.rvsc.2007.05.003. Epub 2007 Jul 9.
Prevalence of anti-Neospora caninum antibodies was measured in serum samples randomly collected from dairy (40 cows from four farms) and beef cattle (120 animals from 12 farms) from the municipality of Santarém, Pará State, Brazil, calculated by using the Win Episcope 2.0 statistical program. The presence of anti-N. caninum antibodies was determined by indirect immunofluorescence-antibody test with a cut-off value of 1:100. We found that 13 farms (81.25%) showed infection rates above 10%, which indicates widespread distribution of N. caninum in the region. The frequency per animal was 19%. No difference was observed between the prevalence values in dairy and beef animals or between farms, which was probably due to the small number of dairy farms examined. The results confirm, for the first time, the presence of anti-N. caninum antibodies in cattle from Pará State and the necessity to further investigate the epidemiology of N. caninum in the Amazon region.
对从巴西帕拉州圣塔伦市随机采集的奶牛(来自四个农场的40头奶牛)和肉牛(来自12个农场的120头牲畜)血清样本中抗犬新孢子虫抗体的流行情况进行了检测,使用Win Episcope 2.0统计程序进行计算。通过间接免疫荧光抗体试验确定抗犬新孢子虫抗体的存在,临界值为1:100。我们发现13个农场(81.25%)的感染率高于10%,这表明犬新孢子虫在该地区分布广泛。每头动物的感染频率为19%。在奶牛和肉牛的流行率值之间或农场之间未观察到差异,这可能是由于检测的奶牛场数量较少。这些结果首次证实了帕拉州牛群中存在抗犬新孢子虫抗体,以及有必要进一步调查亚马逊地区犬新孢子虫的流行病学情况。