Hall C A, Reichel M P, Ellis J T
Novartis Animal Health Australasia Pty Ltd, Yarrandoo R & D Centre, 245 Western Rd, Kemps Creek, NSW 2178, Australia.
Vet Parasitol. 2006 Nov 30;142(1-2):173-8. doi: 10.1016/j.vetpar.2006.06.019. Epub 2006 Jul 31.
To determine the performance characteristics of an Institut Pourquier (IP) enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Neospora caninum in bovine milk and subsequent determination of the prevalence of N. caninum infection in New South Wales (NSW) dairy cattle.
Matching serum and milk samples from 93 cattle were assayed in two commercially available ELISAs for the detection of anti-N. caninum antibodies. Serum test results of one ELISA (IDEXX) were used to determine the N. caninum infection status of the cattle. Optimised cut-off values for the IP ELISA using milk samples were determined by two-graph receiver operating characteristic (TG-ROC) analysis and then applied to a representative sample of 398 milk samples from dairy herds around NSW.
When this ELISA was applied to a representative collection of 398 milk samples from dairy cattle across NSW it demonstrated a 21.1% prevalence of N. caninum infection in those cattle. From the TG-ROC analysis an IP ELISA protocol was derived which suggested a cut-off threshold that would allow milk testing with 97% sensitivity and specificity, respectively, relative to serum testing.
The prevalence of N. caninum in NSW dairy cattle was higher than previously believed. When used on individual milk samples this ELISA demonstrated high sensitivity and specificity and so could be used to accurately identify N. caninum infection. TG-ROC analysis of the IP ELISA optimised the protocol and prescribed cut-off values enabling the ELISA to be used for the screening of N. caninum antibodies in the milk of dairy cattle.
确定Institut Pourquier（IP）酶联免疫吸附测定（ELISA）检测牛乳中抗犬新孢子虫抗体的性能特征，并随后确定新南威尔士州（NSW）奶牛中犬新孢子虫感染的流行率。
对93头牛的血清和乳样进行两种市售ELISA检测，以检测抗犬新孢子虫抗体。使用一种ELISA（IDEXX）的血清检测结果来确定牛的犬新孢子虫感染状况。通过双图受试者工作特征（TG-ROC）分析确定使用乳样的IP ELISA的优化临界值，然后将其应用于来自新南威尔士州各地奶牛场的398份乳样的代表性样本。
当将该ELISA应用于来自新南威尔士州奶牛的398份乳样的代表性样本时，显示这些牛中犬新孢子虫感染的流行率为21.1%。通过TG-ROC分析得出了一种IP ELISA方案，该方案建议了一个临界阈值，相对于血清检测，该阈值可使乳检的灵敏度和特异性分别达到97%。
新南威尔士州奶牛中犬新孢子虫的流行率高于先前的认识。当用于单个乳样时，该ELISA显示出高灵敏度和特异性，因此可用于准确识别犬新孢子虫感染。IP ELISA的TG-ROC分析优化了方案并规定了临界值，使ELISA可用于奶牛乳中犬新孢子虫抗体的筛查。
