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从不同鸟类中分离出的四种传染性法氏囊病病毒的比较。

Comparison of four infectious bursal disease viruses isolated from different bird species.

作者信息

Wang Y S, Wang Z C, Tang Y D, Shi Z L, He K W, Li Y, Hou J B, Yao H C, Fan H J, Lu C P

机构信息

Center for Disease Control and Prevention of Nanjing Command, Huadong Research Institute of Medical Biotechnics, Nanjing, China.

出版信息

Arch Virol. 2007;152(10):1787-97. doi: 10.1007/s00705-007-1022-1. Epub 2007 Jul 6.

Abstract

Four isolates of infectious bursal disease virus (IBDV), isolated from chicken, duck, goose and sparrow in Jiangsu province of China in 2002, were compared. The viruses were stable to the treatments of 60 degrees C for 1 h, pH 2.0 and lipid solvents. Their antigenic relatedness values (R) were from 0.76 to 0.78. Chickens infected with the chicken isolate showed severe clinical symptoms of IBD and the mortality rate was 33.3% (2/6). Chickens infected with the other three viruses survived but their bursas were damaged and the bursa/body-weight ratios were lower than those of the uninfected control (p< 0.01). The titers of anti-IBDV antibody in infected chicken sera reached up to 1600 by virus neutralization and 6400 by ELISA at 10 days post infection. The sequences of the variable region of VP2 were aligned and compared, showing nucleotide variations ranging from 1.5 to 6.7% and deduced aminoacid variations from 0.8 to 2.2%. All had the same heptapeptide, S-W-S-A-S-G-S, Asp279, and Ala284. The four viruses clustered on a phylogenetic tree and were distant from the STC strain. These findings suggested that different bird species naturally infected with IBDV could serve as carriers or reservoirs in IBDV transmission and might play a role in the emergence of variant IBDV.

摘要

对2002年从中国江苏省鸡、鸭、鹅和麻雀中分离出的4株传染性法氏囊病病毒(IBDV)进行了比较。这些病毒在60℃处理1小时、pH 2.0和脂质溶剂处理下均稳定。它们的抗原相关性值(R)为0.76至0.78。感染鸡分离株的鸡表现出严重的传染性法氏囊病临床症状,死亡率为33.3%(2/6)。感染其他三种病毒的鸡存活下来,但它们的法氏囊受损,法氏囊/体重比低于未感染对照(p<0.01)。感染鸡血清中抗IBDV抗体效价在感染后10天通过病毒中和达到1600,通过ELISA达到6400。对VP2可变区序列进行比对和比较,显示核苷酸变异范围为1.5%至6.7%,推导的氨基酸变异范围为0.8%至2.2%。所有病毒都具有相同的七肽S-W-S-A-S-G-S、Asp279和Ala284。这4株病毒在系统发育树上聚类,且与STC株距离较远。这些发现表明,自然感染IBDV的不同鸟类可能作为IBDV传播的携带者或储存宿主,并可能在变异IBDV的出现中发挥作用。

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