Kandelinskaia O L, Topunov A F, Grishchenko E R
Prikl Biokhim Mikrobiol. 2007 May-Jun;43(3):358-65.
Presowing treatment of seeds of lupine of various species and cultivars with brassinosteroids--homobrassinolide and epibrassinolide--caused an increase in protein content and a change in the proportion of some amino acids. Both hormones increased heterogeneity of high- and medium-molecular-weight nonhistone chromatin proteins but had no effect on the polypeptide profile of histones. Using epibrassinolide as an example, we showed that the brassinosteroid-induced increase in protein content in lupine seeds was due primarily to accumulation of the low-molecular-weight components of beta-conglutin. The content of some amino acids in both alpha- and beta-conglutins changed. These changes in protein metabolism correlated with an increase in the content of indoleacetic acid and a decrease in the content of abscisic acid.
用油菜素内酯——高油菜素内酯和表油菜素内酯对不同品种羽扇豆种子进行播前处理,导致蛋白质含量增加以及某些氨基酸比例发生变化。两种激素均增加了高分子量和中分子量非组蛋白染色质蛋白的异质性,但对组蛋白的多肽谱没有影响。以表油菜素内酯为例,我们表明油菜素内酯诱导的羽扇豆种子蛋白质含量增加主要是由于β-伴大豆球蛋白低分子量成分的积累。α-和β-伴大豆球蛋白中某些氨基酸的含量发生了变化。蛋白质代谢的这些变化与吲哚乙酸含量的增加和脱落酸含量的降低相关。