Kawakami Suzue M, Kato Juntaro, Kawakami Shogo, Serizawa Shunsuke
Nishio Nursing College, Togasaki, Nishio, Aichi 445-0074, Japan.
J Plant Res. 2007 Sep;120(5):641-5. doi: 10.1007/s10265-007-0098-8. Epub 2007 Jul 10.
Haploid sporophytes of Osmunda claytoniana (2n = x = 22) were apogamously produced from calli when cultivated on a hormone-free medium. Flow cytometric analysis showed that ploidy chimeras were spontaneously produced in a haploid sporophyte of O. claytoniana and those of O. japonica that were obtained in the previous study. In the haploid sporophyte of O. claytoniana, a diploid pinnule and a partially diploid terminal segment were produced in a haploid pinna. In O. japonica, a haploid sporophyte yielded a diploid pinna in a haploid frond, and another haploid sporophyte yielded a diploid pinnule in a haploid pinna. Diploid chimeras were large in size and could be readily distinguished from other haploid parts of the fronds. It is likely that the chimeras were produced clonally from a single diploid cell that established chromosome doubling.
在无激素培养基上培养时，从愈伤组织中无融合生殖地产生了紫萁（2n = x = 22）的单倍体孢子体。流式细胞术分析表明，在紫萁的单倍体孢子体以及在前一项研究中获得的日本紫萁的单倍体孢子体中自发产生了倍性嵌合体。在紫萁的单倍体孢子体中，单倍体羽片中产生了二倍体小羽片和部分二倍体的顶端节段。在日本紫萁中，一个单倍体孢子体在单倍体叶中产生了二倍体羽片，另一个单倍体孢子体在单倍体羽片中产生了二倍体小羽片。二倍体嵌合体体积较大，很容易与叶的其他单倍体部分区分开来。这些嵌合体很可能是由一个建立了染色体加倍的单个二倍体细胞克隆产生的。
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