Namkung J-H, Lee J-E, Kim E, Cho H-J, Kim S, Shin E-S, Cho E-Y, Yang J-M
Department of Dermatology, Sungkyunkwan University School of Medicine, Samsung Medical Center, Seoul, Korea.
Allergy. 2007 Aug;62(8):934-42. doi: 10.1111/j.1398-9995.2007.01445.x.
Eosinophils are recruited into the affected tissue of asthma and atopic dermatitis (AD) patients. IL-5 and IL-5R are highly expressed in the AD skin lesions, yet the reported levels of IL-8 are controversial.
We genotyped 17 singlenucleotide polymorphisms (SNPs) from five genes of the 1120 case-control samples (646 AD and 474 controls). We measured the serum IL-5 concentrations in 87 individuals [36 ADe (AD extrinsic), 18 ADi (AD intrinsic) and 33 controls] by ELISA, and compared the results among these groups.
The rs2522411SNP and haplotype T-A in the IL-5 gene were significantly associated with the ADe. The serum IL-5 concentration was higher in the ADe than that in the ADi patients without any correlation with the rs2522411SNP. In the IL-5RA gene, the rs334809SNP showed a weak association with AD, and the rs6771148SNP and the haplotype T-C-T of the three adjacent tagged SNPs had an effect on the blood eosinophil counts and the serum ECP levels in the AD patients. However, we could not detect any relationship between AD and the SNPs in the IL-8 and IL-8R genes.
We found that the rs2522411SNP and the haplotype T-A in the IL-5 gene and the serum IL-5 levels were strongly associated with the allergic type of AD, but not with the nonallergic type of AD. The association of the rs6771148SNP and the haplotype T-C-T in the IL5RA gene with the blood eosinophil counts and the serum ECP levels indicates that the IL5RA gene has a role for controlling eosinophils in the peripheral blood.
嗜酸性粒细胞会被招募到哮喘和特应性皮炎（AD）患者的病变组织中。白细胞介素-5（IL-5）和白细胞介素-5受体（IL-5R）在AD皮肤病变中高表达，但关于白细胞介素-8（IL-8）的报道水平存在争议。
我们对1120例病例对照样本（646例AD患者和474例对照）的五个基因中的17个单核苷酸多态性（SNP）进行了基因分型。我们通过酶联免疫吸附测定（ELISA）测量了87名个体[36例ADe（外源性AD）、18例ADi（内源性AD）和33例对照]的血清IL-5浓度，并比较了这些组之间的结果。
IL-5基因中的rs2522411SNP和单倍型T-A与ADe显著相关。ADe患者的血清IL-5浓度高于ADi患者，且与rs2522411SNP无相关性。在IL-5RA基因中，rs334809SNP与AD呈弱关联，rs6771148SNP以及三个相邻标签SNP的单倍型T-C-T对AD患者的血液嗜酸性粒细胞计数和血清嗜酸性粒细胞阳离子蛋白（ECP）水平有影响。然而，我们未检测到AD与IL-8和IL-8R基因中的SNP之间存在任何关系。
我们发现IL-5基因中的rs2522411SNP和单倍型T-A以及血清IL-5水平与过敏性AD密切相关，而与非过敏性AD无关。IL5RA基因中的rs6771148SNP和单倍型T-C-T与血液嗜酸性粒细胞计数和血清ECP水平的关联表明，IL5RA基因在控制外周血嗜酸性粒细胞方面发挥作用。
