Zhang H M, Bacon L D, Heidari M, Muir W M, Groenen M A M, Zhang Y, Wong G K S, Fulton J E, O'Sullivan N P, Albers G A A, Vereijken A L J, Rattink A P, Okimoto R, McKay J C, McLeod S, Cheng H H
USDA, ARS, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA.
Avian Pathol. 2007 Aug;36(4):283-91. doi: 10.1080/03079450701449248.
The tumour virus B (TVB) locus encodes cellular receptors mediating infection by three subgroups of avian leukosis virus (B, D, and E). Three major alleles, TVBS1, TVBS3, and TVBR, have been described. TVBS1 encodes a cellular receptor mediating infection of subgroups B, D, and E. TVBS3 encodes the receptor for two subgroups, B and D, and TVBR encodes a dysfunctional receptor that does not permit infection by any of the subgroups, B, D, or E. Genetic diversity at the TVB locus of chickens was investigated in both layer and broiler commercial pure lines and laboratory lines. Genotyping assays were developed for both medium-throughput and high-throughput analysis. Of the 36 broiler lines sampled, 14 were fixed for the susceptible allele TVBS1. Across all broiler lines, 83% of chickens were typed as TVBS1/S1, 3% as TVBR/R, and 14% as TVBS1/R. In the egg-layer lines, five of the 16 tested were fixed for TVBS1/S1. About 44% of egg-layers were typed as TVBS1/S1, 15% as TVBR/R, with the rest segregating for two or three of the alleles. In the laboratory chickens, 60% were fixed for TVBS1/S1, 6% for TVBS3/S3, 14% for TVBR/R, and the rest were heterozygotes (TVBS1/S3 or TVBS1/R). All commercial pure lines examined in this study carry the TVBS1 allele that sustains the susceptibility to avian leukosis viruses B, D, and E. More importantly, the TVB*R allele was identified in multiple populations, thus upholding the opportunities for genetic improvement through selection.
肿瘤病毒B（TVB）基因座编码介导禽白血病病毒三个亚群（B、D和E）感染的细胞受体。已描述了三个主要等位基因，即TVBS1、TVBS3和TVBR。TVBS1编码介导B、D和E亚群感染的细胞受体。TVBS3编码B和D两个亚群的受体，而TVBR编码一种功能失调的受体，不允许B、D或E任何一个亚群感染。在蛋鸡和肉鸡商业纯系以及实验系中研究了鸡TVB基因座的遗传多样性。开发了用于中通量和高通量分析的基因分型检测方法。在抽样的36个肉鸡品系中，有14个对易感等位基因TVBS1固定。在所有肉鸡品系中，83%的鸡被分型为TVBS1/S1，3%为TVBR/R，14%为TVBS1/R。在蛋鸡品系中，16个测试品系中有5个对TVBS1/S1固定。约44%的蛋鸡被分型为TVBS1/S1，15%为TVBR/R，其余的在两个或三个等位基因间分离。在实验鸡中，60%对TVBS1/S1固定，6%对TVBS3/S3固定，14%对TVBR/R固定，其余为杂合子（TVBS1/S3或TVBS1/R）。本研究中检测的所有商业纯系都携带TVBS1等位基因，该等位基因使鸡对禽白血病病毒B、D和E易感。更重要的是，在多个群体中鉴定出了TVB*R等位基因，从而为通过选择进行遗传改良提供了机会。
