Ozcan K, Ozen H, Karaman M
University of Kafkas, Faculty of Veterinary Medicine, Department of Pathology, Kars, Turkey.
Avian Pathol. 2007 Aug;36(4):325-9. doi: 10.1080/03079450701460757.
Three naturally ethylene glycol (EG)-intoxicated geese were investigated for pathological changes, nitrosative tissue damage and apoptotic cell death. Severe degeneration of kidney tubular epithelium and congestion of kidney and liver tissues were observed. Immunohistochemical staining for inducible nitric oxide synthase and nitrotyrosine revealed strong immunoreactivity with both antibodies in kidney and liver tissues compared with the weak immunostaining in the control animals. In both tissues of the EG-intoxicated geese, erythrocytes were also highly immunoreactive with nitrotyrosine antibody. A high degree of apoptotic cell death was present in the kidney tubule epithelium of EG-intoxicated geese. Some apoptotic cells were also observed in the liver. These results show that nitrosative tissue damage and apoptotic cell death takes place in kidney and liver during EG intoxication in geese.
对三只自然中毒的乙二醇（EG）的鹅进行了病理变化、亚硝化组织损伤和凋亡性细胞死亡的研究。观察到肾小管上皮严重变性以及肾脏和肝脏组织充血。诱导型一氧化氮合酶和硝基酪氨酸的免疫组织化学染色显示，与对照动物的弱免疫染色相比，肾脏和肝脏组织中两种抗体均有强免疫反应性。在乙二醇中毒鹅的两种组织中，红细胞对硝基酪氨酸抗体也有高度免疫反应性。乙二醇中毒鹅的肾小管上皮存在高度凋亡性细胞死亡。在肝脏中也观察到一些凋亡细胞。这些结果表明，在鹅乙二醇中毒期间，肾脏和肝脏发生了亚硝化组织损伤和凋亡性细胞死亡。
