Elias Mikael, Dupuy Jérôme, Merone Luigia, Lecomte Claude, Rossi Mosè, Masson Patrick, Manco Giuseppe, Chabriere Eric
Laboratoire de Cristallographie et Modélisation des Matériaux Minéraux et Biologiques, CNRS-Université Henri Poincaré, 54506 Vandoeuvre-lès-Nancy, France.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):553-5. doi: 10.1107/S1744309107023512. Epub 2007 Jun 11.
Organophosphates constitute the largest class of insecticides used worldwide and some of them are potent nerve agents. Consequently, organophosphate-degrading enzymes are of paramount interest as they could be used as bioscavengers and biodecontaminants. Phosphotriesterases (PTEs) are capable of hydrolyzing these toxic compounds with high efficiency. A distant and hyperthermophilic representative of the PTE family was cloned from the archeon Sulfolobus solfataricus MT4, overexpressed in Escherichia coli and crystallized; the crystals diffracted to 2.54 A resolution. Owing to its exceptional thermostability, this PTE may be an excellent candidate for obtaining an efficient organophosphate biodecontaminant. Here, the crystallization conditions and data collection for the hyperthermophilic S. solfataricus PTE are reported.
有机磷酸酯是全球使用的最大一类杀虫剂，其中一些是强效神经毒剂。因此，有机磷酸酯降解酶备受关注，因为它们可用作生物清除剂和生物去污剂。磷酸三酯酶（PTEs）能够高效水解这些有毒化合物。从嗜热古菌嗜热栖热菌MT4中克隆了PTE家族的一个远源且嗜热的代表，在大肠杆菌中进行了过表达并结晶；晶体衍射分辨率达到2.54 Å。由于其卓越的热稳定性，这种PTE可能是获得高效有机磷酸酯生物去污剂的极佳候选者。本文报道了嗜热栖热菌PTE的结晶条件和数据收集情况。
