Horne Irene, Qiu Xinghui, Russell Robyn J, Oakeshott John G
CSIRO Entomology, GPO Box 1700, Canberra, ACT 2601, Australia. irene.horne@
FEMS Microbiol Lett. 2003 May 16;222(1):1-8. doi: 10.1016/S0378-1097(03)00211-8.
We report a transposase gene (tnpA) upstream of the opdA phosphotriesterase gene of Agrobacterium radiobacter P230, as well as inverted repeats indicative of insertion sequences, flanking the two genes. Both the tnpA gene and the inverted repeats resemble the Tn610 transposon from Mycobacterium fortuitum. Two additional putative open reading frames separate opdA and tnpA with inferred translation products with similarity to two proteins encoded on the Geobacillus stearothermophilus IS5376 transposon. To test the proposition that these genes were contained on a transposon, an artificial composite transposon was constructed. This artificial transposon was then delivered into Escherichia coli DH10beta cells. Transposition was demonstrated by the presence of opdA on the E. coli chromosome and confirmation of insertion by inverse polymerase chain reaction. The data presented suggest a possible role of transposition in the distribution of the opd/opdA genes across a wide range of soil bacteria.
我们报道了放射土壤杆菌P230的opdA磷酸三酯酶基因上游的一个转座酶基因（tnpA），以及这两个基因两侧指示插入序列的反向重复序列。tnpA基因和反向重复序列都类似于来自偶然分枝杆菌的Tn610转座子。另外两个推定的开放阅读框将opdA和tnpA分开，其推断的翻译产物与嗜热栖热放线菌IS5376转座子上编码的两种蛋白质相似。为了验证这些基因存在于转座子上这一推测，构建了一个人工复合转座子。然后将这个人工转座子导入大肠杆菌DH10β细胞。通过在大肠杆菌染色体上检测到opdA以及通过反向聚合酶链反应确认插入来证明转座作用。所呈现的数据表明转座可能在opd/opdA基因在广泛的土壤细菌中的分布中发挥作用。
