Yasumuro Y, Tomita Y, Meguro T, Taki M, Itoh H, Yamada K
Clinical Laboratory, St. Marianna University School of Hospital, Kawasaki.
Rinsho Byori. 1991 Nov;39(11):1209-14.
Further modification of the previously reported peroxidase staining method for von Willebrand factor (vWF) multimer is described in this article. The alteration are as follows: affinity purified anti-human vWF rabbit IgG was replaced by commercial anti-human vWF rabbit IgG, and washing and reaction steps were shortened markedly. Despite these modification, the resolution of vWF multimers on the nitrocellulose membrane remains comparable to the autoradiography. However, unlike autoradiography, results produced by this method can be obtained in a day. The features of this method are especially useful in clinical laboratories where both time and equipments are limited.
本文描述了对先前报道的用于血管性血友病因子（vWF）多聚体的过氧化物酶染色方法的进一步改进。改变如下：用市售抗人vWF兔IgG替代亲和纯化的抗人vWF兔IgG，并显著缩短洗涤和反应步骤。尽管有这些改进，但硝酸纤维素膜上vWF多聚体的分辨率仍与放射自显影相当。然而，与放射自显影不同的是，该方法产生的结果可在一天内获得。该方法的特点在时间和设备都有限的临床实验室中特别有用。
