[用柠檬酸裂解酶对血清柠檬酸进行酶法测定]

[An enzymatic determination of serum citrate with citrate lyase].

作者信息

Yasukawa S, Ebisuno S, Morimoto S, Uehara M, Ohkawa T

机构信息

Department of Urology, Wakayama Medical College.

出版信息

Nihon Hinyokika Gakkai Zasshi. 1991 Nov;82(11):1748-53. doi: 10.5980/jpnjurol1989.82.1748.

DOI:10.5980/jpnjurol1989.82.1748

PMID:1762264

Abstract

A method for the enzymatic determination of the serum citrate using citrate lyase is described. The optimum pH was equal to or higher than 8.6. Prior to the determination of the serum citrate, the samples were deproteinized with perchloric acid. After the precipitation of the proteins, potassium hydroxide was added under an ice-cooling condition in order to neutralize the solution. The recovery rates of the citrate added to serum ranged from 92% to 108%, and the coefficient of variation of triplicate assay was 4.2%. The mean serum value of 17 healthy subjects was 1.57 mg/dl, with a range of 1.03-2.03 mg/dl. In seven healthy subjects, 3 g of sodium-potassium citrate was administered orally and the serum citrate was measured 0, 15, 30, 45, 60, and 120 min later. The serum citrate was significantly increased in the periods between 15 and 60 min.

摘要

描述了一种使用柠檬酸裂解酶酶促测定血清柠檬酸的方法。最佳pH等于或高于8.6。在测定血清柠檬酸之前，用高氯酸对样品进行脱蛋白处理。蛋白质沉淀后，在冰冷却条件下加入氢氧化钾以中和溶液。添加到血清中的柠檬酸回收率在92%至108%之间，三次重复测定的变异系数为4.2%。17名健康受试者的血清平均值为1.57mg/dl，范围为1.03 - 2.03mg/dl。在7名健康受试者中，口服3g柠檬酸钾钠，分别在0、15、30、45、60和120分钟后测量血清柠檬酸。在15至60分钟期间血清柠檬酸显著升高。