高效液相色谱法同时测定雷公藤提取物中的四种倍半萜生物碱
Simultaneous determination of four sesquiterpene alkaloids in Tripterygium wilfordii Hook. F. extracts by high-performance liquid chromatography.
作者信息
Ouyang Xiao-Kun, Jin Mi-Cong, He Chao-Hong
机构信息
Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, People's Republic of China.
出版信息
Phytochem Anal. 2007 Jul-Aug;18(4):320-5. doi: 10.1002/pca.985.
Wilfortrine, wilfordine, wilforgine and wilforine are four major bioactive sesquiterpene alkaloids in Tripterygium wilfordii Hook. F. The first analytical determination of the four major bioactive alkaloids is described. The four alkaloids are well-resolved within 15 min using the developed HPLC method. The identity of the analytes was confirmed by an HPLC-MS experiment, with all compounds being clearly assignable by atmospheric pressure chemical ionization (APCI) positive mode analysis. The method was validated for limit of qualification, linearity and inter-day variation of precision and accuracy. Seven T. wilfordii samples (extracts and commercial product) were successfully analysed.
雷公藤碱、雷公藤定碱、雷公藤春碱和雷公藤辛碱是雷公藤中四种主要的具有生物活性的倍半萜生物碱。本文描述了这四种主要生物活性生物碱的首次分析测定。使用所开发的高效液相色谱法，这四种生物碱在15分钟内得到了很好的分离。通过高效液相色谱 - 质谱实验确认了分析物的身份，所有化合物通过大气压化学电离（APCI）正模式分析均可明确归属。该方法在定性限、线性以及精密度和准确度的日间变化方面进行了验证。成功分析了七个雷公藤样品（提取物和商业产品）。
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