Leite Aline de Lima, Santiago Joel Ferreira, Levy Flavia Mauad, Maria Andrea Gutierrez, Fernandes Mileni da Silva, Salvadori Daisy Maria Favero, Ribeiro Daniel Araki, Buzalaf Marilia Afonso Rabelo
Department of Biological Sciences, Bauru Dental School, University of São Paulo, USP, 17012-901 Bauru, SP, Brazil.
Hum Exp Toxicol. 2007 May;26(5):435-40. doi: 10.1177/0960327107076288.
Fluoride has been widely used in dentistry as a caries prophylactic agent. However, there has been some speculation that excess fluoride could cause an impact on genome integrity. In the current study, the potential DNA damage associated with exposure to fluoride was assessed in cells of blood, liver, kidney, thyroid gland and urinary bladder by the single cell gel (comet) assay. Male Wistar rats aging 75 days were distributed into seven groups: Groups 1 (control), 2, 3, 4, 5, 6 and 7 received 0 (deionized water), 10, 20, 40, 60, 80 and 100 mgF/Kg body weight from sodium fluoride (NaF), respectively, by gastrogavage. These groups were killed at 2 h after the administration of the fluoride doses. The level of DNA strand breaks did not increase in all organs evaluated and at all doses of NaF tested, as depicted by the mean tail moment. Taken together, our results suggest that oral exposure to NaF did not result in systemic genotoxic effect in multiple organs related to fluoride toxicity. Since DNA damage is an important step in events leading to carcinogenesis, this study represents a relevant contribution to the correct evaluation of the potential health risk associated with chemical exposure.
氟化物作为一种龋齿预防剂已在牙科领域广泛应用。然而，有人猜测过量的氟化物可能会对基因组完整性产生影响。在本研究中，通过单细胞凝胶（彗星）试验评估了血液、肝脏、肾脏、甲状腺和膀胱细胞中与氟化物暴露相关的潜在DNA损伤。将75日龄的雄性Wistar大鼠分为七组：第1组（对照组）、第2、3、4、5、6和7组分别通过灌胃给予0（去离子水）、10、20、40、60、80和100mgF/千克体重的氟化钠（NaF）。在给予氟化物剂量2小时后处死这些组。如平均尾矩所示，在所有评估的器官和所有测试的NaF剂量下，DNA链断裂水平均未增加。综上所述，我们的结果表明，口服NaF不会导致与氟化物毒性相关的多个器官产生全身性遗传毒性作用。由于DNA损伤是导致致癌作用的重要步骤，本研究为正确评估与化学物质暴露相关的潜在健康风险做出了相关贡献。
