Institoris L, Banfi H, Lengyel Zs, Papp A, Nagymajtenyi L
Department of Public Health, Faculty of Medicine, University of Szeged, Dóm tér 10, H-6720 Szeged, Hungary.
Hum Exp Toxicol. 2007 May;26(5):441-5. doi: 10.1177/0960327107076887.
The effects of amitraz, a formamidine pesticide, were investigated in four-week old outbred male Wistar rats on certain classic toxicological and haematological parameters as well as on specific immune functions. The animals were treated, per os by gavage for 28 days, in a five-day treatment two days break system, with 26.5, 21.1, 10.6 and 5.29 mg/kg/day amitraz. On the 29th day, organ weights of the thymus, heart, lung, spleen, liver, kidneys, adrenals, testicles and popliteal lymph node; WBC and RBC counts, Ht, MCV, haemoglobin; and cell content of the femoral bone marrow were determined. In two separate groups, the effects of amitraz on the PFC content of the spleen, and on the maximum level and time course of DTH reaction, were investigated. Amitraz in 26.5 mg/kg dose increased relative adrenal weight, and decreased relative liver weight, MCV value, PFC content of the spleen, and the maximum level of DTH reaction. The 21.1 mg/kg dose decreased only MCV value, while 10.6 mg/kg elevated the liver-to-brain weight ratio. Based of these findings, a NOEL dose of 5.29 mg/kg was determined for amitraz in this experimental system; while the LOEL doses were 10.6 mg/kg for the general toxicological, 21.1 mg/kg for the haematological and 26.5 mg/kg for the immune function parameters. The results show that the exposure sensitivity of these immune functions to amitraz is lower than that of some other toxicological and haematological parameters.
研究了甲脒类杀虫剂双甲脒对4周龄远交系雄性Wistar大鼠某些经典毒理学和血液学参数以及特定免疫功能的影响。动物采用经口灌胃给药，按照5天给药、2天休息的方式，连续给药28天，剂量分别为26.5、21.1、10.6和5.29mg/kg/天的双甲脒。在第29天，测定胸腺、心脏、肺、脾脏、肝脏、肾脏、肾上腺、睾丸和腘窝淋巴结的器官重量；白细胞和红细胞计数、血细胞比容、平均红细胞体积、血红蛋白；以及股骨骨髓的细胞含量。在另外两组中，研究了双甲脒对脾脏空斑形成细胞含量以及迟发型超敏反应的最大水平和时间进程的影响。26.5mg/kg剂量的双甲脒增加了肾上腺相对重量，降低了肝脏相对重量、平均红细胞体积值、脾脏空斑形成细胞含量以及迟发型超敏反应的最大水平。21.1mg/kg剂量仅降低了平均红细胞体积值，而10.6mg/kg剂量提高了肝脑重量比。基于这些发现，在该实验系统中确定双甲脒的无观察到有害作用水平（NOEL）剂量为5.29mg/kg；而最低观察到有害作用水平（LOEL）剂量，一般毒理学参数为10.6mg/kg，血液学参数为21.1mg/kg，免疫功能参数为26.5mg/kg。结果表明，这些免疫功能对双甲脒的暴露敏感性低于其他一些毒理学和血液学参数。
