Kimmel Robert, Icreverzi Amalia, Neiman Paul
Fred Hutchinson Cancer Research Center, Seattle, Washington, USA.
Subcell Biochem. 2006;40:363-71. doi: 10.1007/978-1-4020-4896-8_27.
Genome-wide assessment of DNA gains and losses can be accomplished by comparative hybridization using a variety of microarray platforms that employ oligo, cDNA, BAC and other sequences as probes. Here we describe the preparation of genomic DNA for hybridization to a set of chicken cDNA probes spotted on glass slide microarrays. Method 1 can be used to assess DNA copy-number differences between two genomes, typically an experimental genome and a normal, diploid genome. We then present a specialized application of array CGH for detecting DNA amplifications containing long, inverted repeat structures (palindromes). Method 2 describes the procedure for palindrome enrichment and the internal controls used to distinguish direct versus inverted, long repeat structures using the cDNA microarray platform.
通过使用多种以寡核苷酸、cDNA、BAC和其他序列作为探针的微阵列平台进行比较杂交，可实现全基因组范围内DNA增减的评估。在此，我们描述了用于与点样在玻片微阵列上的一组鸡cDNA探针杂交的基因组DNA的制备方法。方法1可用于评估两个基因组之间的DNA拷贝数差异，通常是实验基因组与正常二倍体基因组之间的差异。然后，我们介绍了用于检测包含长反向重复结构（回文序列）的DNA扩增的阵列比较基因组杂交（array CGH）的一种特殊应用。方法2描述了使用cDNA微阵列平台进行回文序列富集的程序以及用于区分正向与反向长重复结构的内部对照。
