Gil-Salas F M, Morris J, Colyer A, Budge G, Boonham N, Cuadrado I M, Janssen D
Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Alimentaria y de la Producción Ecológica (I.F.A.P.A., C.I.C.E.), Junta de Andalucía, 04745 La Mojonera, Almeria, Spain.
J Virol Methods. 2007 Dec;146(1-2):45-51. doi: 10.1016/j.jviromet.2007.05.032. Epub 2007 Jul 12.
Reverse transcription followed by real-time PCR assays based on TaqMan chemistry have been developed for the detection and quantification of Cucumber vein yellowing virus (CVYV) and Cucurbit yellow stunting disorder virus (CYSDV) in individual adults of the whitefly vector Bemisia tabaci. The method includes an internal control for the detection of a gene from B. tabaci to compensate for variations in extraction efficiency. The assays designed were used to estimate proportions of viruliferous whiteflies collected from commercial greenhouse-grown crops in Spain. In a significant number of whiteflies, both viruses were detected and their amounts were estimated. The assays could be used to assist risk assessment of CVYV and CYSDV which constitute limiting factors in cucurbit crops. They are also suited to investigating the epidemiology and plant-virus-vector relationships in these diseases.
基于TaqMan化学的逆转录实时PCR检测方法已被开发出来，用于检测和定量烟粉虱传播媒介中黄瓜叶脉黄化病毒（CVYV）和葫芦科黄化矮化病毒（CYSDV）的个体成虫。该方法包括一个用于检测烟粉虱基因的内部对照，以补偿提取效率的差异。所设计的检测方法用于估计从西班牙商业温室种植作物中采集的带毒烟粉虱的比例。在大量烟粉虱中，检测到了两种病毒并估计了它们的数量。这些检测方法可用于协助对构成葫芦科作物限制因素的CVYV和CYSDV进行风险评估。它们也适用于研究这些疾病的流行病学以及植物-病毒-媒介关系。
