Nawrot Robert, Lesniewicz Krzysztof, Pienkowska Joanna, Gozdzicka-Jozefiak Anna
Department of Molecular Virology, Institute of Experimental Biology, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland.
Fitoterapia. 2007 Dec;78(7-8):496-501. doi: 10.1016/j.fitote.2007.04.012. Epub 2007 May 25.
Using affinity chromatography, SDS-PAGE, peroxidase activity assay and mass spectrometry data, a new extracellular peroxidase (CMP) from Chelidonium majus milky sap was isolated and characterized. The protein has a molecular weight of about 40 kDa and belongs to secretory class III plant peroxidases. The peroxidase activity is also accompanied by DN-ase activities. A novel CMP combined with other proteins is probably involved in development and differentiation of the plant and defence against different pathogens. It suggests that the biological activity of C. majus whole plants and extracts may depend not only on its alkaloidal content but also on the presence of biologically active proteins.
利用亲和色谱、SDS - 聚丙烯酰胺凝胶电泳、过氧化物酶活性测定和质谱数据，从白屈菜乳状汁液中分离并鉴定出一种新的细胞外过氧化物酶（CMP）。该蛋白质分子量约为40 kDa，属于分泌型III类植物过氧化物酶。过氧化物酶活性还伴随着DNA酶活性。一种与其他蛋白质结合的新型CMP可能参与植物的发育和分化以及对不同病原体的防御。这表明白屈菜全株及其提取物的生物活性可能不仅取决于其生物碱含量，还取决于生物活性蛋白质的存在。
