Piao Zhenzi, Toyota-Hanatani Yukiko, Ohta Hiroaki, Sasai Kazumi, Tani Hiroyuki, Baba Eiichiroh
Department of Veterinary Internal Medicine, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka, Japan.
Vet Microbiol. 2007 Nov 15;125(1-2):111-9. doi: 10.1016/j.vetmic.2007.05.008. Epub 2007 May 18.
Levels of Salmonella enterica subsp. enterica serovar Enteritidis infection and serum S. Enteritidis antibodies after experimental S. Enteritidis challenge and feed withdrawal were investigated in S. Enteritidis-vaccinated and unvaccinated hens. The results were used to determine whether formalin-inactivated S. Enteritidis vaccination can protect layer hens from S. Enteritidis challenge during feed withdrawal periods. S. Enteritidis infection rates were evaluated from cloacal swabs, eggs and organs. Serum antibody titers to deflagellated S. Enteritidis whole cells (DEWC) and S. Enteritidis FliC-specific 9-kDa polypeptide (SEp 9) were examined by commercial ELISA kits. Cloacal S. Enteritidis recovery rates were lower in the vaccinated than unvaccinated group. Recovery rates of S. Enteritidis from samples increased after feed withdrawal and decreased after re-introduction of feed. S. Enteritidis counts in cloacal swabs were lower in the vaccinated than in the unvaccinated group (P<0.05). More S. Enteritidis-positive eggs were detected from the unvaccinated group. Before S. Enteritidis challenge, the DEWC ELISA titer of the vaccinated group was higher (P<0.05) than the unvaccinated group; subsequently, the S. Enteritidis DEWC ELISA titers of both groups increased gradually. In contrast, only the vaccinated group elicited high SEp-9 antibody titer during post-challenge and feed withdrawal. Additionally, vaccinated hens yielded negative S. Enteritidis isolation rates from egg contents. There is a correlation between negative S. Enteritidis isolation rates and high SEp 9 titers in vaccinated layer hens challenged with S. Enteritidis and subjected to feed withdrawal regimens. These findings suggest the S. Enteritidis vaccination of pullets may protect against S. Enteritidis infection during forced molting and that SEp 9 titer could be a potential indicator of antibody protection against S. Enteritidis infection. The potential of the SEp 9 peptide as an antigen for S. Enteritidis vaccination in the future is worth noting.
在接种肠炎沙门氏菌疫苗和未接种疫苗的母鸡中，研究了实验性肠炎沙门氏菌攻击和停喂后肠炎沙门氏菌亚种肠炎血清型肠炎沙门氏菌的感染水平和血清肠炎沙门氏菌抗体。研究结果用于确定甲醛灭活的肠炎沙门氏菌疫苗接种能否在停喂期间保护蛋鸡免受肠炎沙门氏菌攻击。通过泄殖腔拭子、鸡蛋和器官评估肠炎沙门氏菌感染率。使用商用ELISA试剂盒检测针对去鞭毛肠炎沙门氏菌全细胞（DEWC）和肠炎沙门氏菌鞭毛蛋白特异性9-kDa多肽（SEp 9）的血清抗体滴度。接种疫苗组的泄殖腔肠炎沙门氏菌回收率低于未接种疫苗组。停喂后样本中肠炎沙门氏菌的回收率增加，重新投喂后降低。接种疫苗组泄殖腔拭子中的肠炎沙门氏菌计数低于未接种疫苗组（P<0.05）。未接种疫苗组检测到更多肠炎沙门氏菌阳性鸡蛋。在肠炎沙门氏菌攻击前，接种疫苗组的DEWC ELISA滴度高于未接种疫苗组（P<0.05）；随后，两组的肠炎沙门氏菌DEWC ELISA滴度逐渐升高。相比之下，只有接种疫苗组在攻击后和停喂期间引发了高SEp-9抗体滴度。此外，接种疫苗的母鸡蛋内容物的肠炎沙门氏菌分离率为阴性。在接受肠炎沙门氏菌攻击并采用停喂方案的接种疫苗蛋鸡中，肠炎沙门氏菌阴性分离率与高SEp 9滴度之间存在相关性。这些发现表明，雏鸡接种肠炎沙门氏菌疫苗可能在强制换羽期间预防肠炎沙门氏菌感染，并且SEp 9滴度可能是抗体预防肠炎沙门氏菌感染的潜在指标。未来，SEp 9肽作为肠炎沙门氏菌疫苗接种抗原的潜力值得关注。
