Nakamura M, Nagamine N, Takahashi T, Suzuki S, Sato S
National Veterinary Assay Laboratory, Tokyo, Japan.
Avian Dis. 1994 Oct-Dec;38(4):717-24.
An oil-emulsion vaccine was prepared from phage type 4 Salmonella enteritidis to assess the potential protective efficacy of S. enteritidis vaccine and the effect of stress after vaccination. Hens were vaccinated at 14 and 18 weeks of age and challenged orally at 24 weeks of age with 10(6) or 10(3) cells of a homologous S. enteritidis strain. Pullets were vaccinated at 8 and 12 weeks of age and challenged with 10(9) cells. The O antibody titers of vaccinated hens rose quickly and were unchanged after challenge. S. enteritidis was isolated from cecal droppings of significantly fewer vaccinated hens than unvaccinated control hens for 6-21 days and 2-15 days after challenge with 10(6) and 10(3) cells, respectively. S. enteritidis was also isolated from livers and spleens of significantly fewer vaccinated pullets for 1 week. Vaccinated and control hens were equally affected by exposure to environmental stress, the result being that the shedding rate of S. enteritidis in cecal droppings increased equally in both groups.
制备了一种由4型肠炎沙门氏菌制成的油乳剂疫苗，以评估肠炎沙门氏菌疫苗的潜在保护效力以及接种疫苗后应激的影响。母鸡在14周龄和18周龄时接种疫苗，并在24周龄时口服接种10⁶或10³个同源肠炎沙门氏菌菌株的细胞进行攻毒。小母鸡在8周龄和12周龄时接种疫苗，并接种10⁹个细胞进行攻毒。接种疫苗的母鸡的O抗体滴度迅速上升，攻毒后保持不变。分别用10⁶和10³个细胞攻毒后，在6至21天和2至15天内，从接种疫苗的母鸡盲肠粪便中分离出肠炎沙门氏菌的数量明显少于未接种疫苗的对照母鸡。在1周内，从接种疫苗的小母鸡的肝脏和脾脏中分离出肠炎沙门氏菌的数量也明显较少。接种疫苗的母鸡和对照母鸡受到环境应激的影响相同，结果两组盲肠粪便中肠炎沙门氏菌的排出率均同等增加。
