作为抗狂犬病药物的短干扰RNA
Short-interfering RNAs as antivirals against rabies.
作者信息
Brandão Paulo Eduardo, Castilho Juliana Galera, Fahl Willian, Carnieli Pedro, Oliveira Rafael de Novaes, Macedo Carla Isabel, Carrieri Maria Luiza, Kotait Ivanete
机构信息
School of Veterinary Medicine, University of São Paulo, Avenida Professor Doutor Orlando Marques de Paiva 87, 05508-000 São Paulo, SP, Brazil.
出版信息
Braz J Infect Dis. 2007 Apr;11(2):224-5. doi: 10.1590/s1413-86702007000200011.
This study aimed to test in vitro a RNA-interference based antiviral approach for rabies with short-interfering RNAs (siRNAs) against rabies virus nucleoprotein mRNA. BHK-21 cells were infected with serial dilutions of PV rabies virus strain and transfected with a pool of three siRNAs. Direct immunofluorescence staining showed a 5-time decrease in virus titer when compared to a non-treated plate, showing a promising new approach to the development of antivirals for rabies treatment.
本研究旨在体外测试一种基于RNA干扰的狂犬病抗病毒方法，该方法使用针对狂犬病病毒核蛋白mRNA的小干扰RNA（siRNA）。用PV狂犬病病毒株的系列稀释液感染BHK-21细胞，并转染三种siRNA的混合物。直接免疫荧光染色显示，与未处理的平板相比，病毒滴度降低了5倍，这表明一种有前景的狂犬病治疗抗病毒药物开发新方法。
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