Janovy J, Bolek M G, Detwiler J, Schwank S, Knipes A, Langford G J
School of Biological Sciences, University of Nebraska Lincoln, Lincoln, Nebraska 68588-0118, USA.
J Parasitol. 2007 Jun;93(3):714-6. doi: 10.1645/GE-1078R.1.
The surface architecture of oocysts produced by Gregarina niphandrodes (Eugregarinorida) from Tenebrio molitor adults (Coleoptera: Tenebrionidae) as revealed by scanning electron microscopy is reported. Gametocysts were allowed to dehisce on 15-mm, round cover glasses; the cover glasses with their oocysts chains were then mounted on stubs without further processing, and sputter-coated with 20-nm gold palladium. Scanning electron microscopy was performed at 10-15 kV with a Hitachi 3000N SEM. Oocysts retained their characteristic shapes as reported in the original species description but showed longitudinal ridges of relatively uniform height, width, and spacing, in separate fields on either side of a central equatorial bulge in the oocysts. There was no ultrastructural evidence of an enclosing external sheath holding the oocysts in a chain. Oocyst ends were flared slightly, and the chain itself was twisted, with adjacent oocysts offset slightly from one another. This article now provides an additional set of structural characters potentially useful in gregarine systematics.
本文报道了通过扫描电子显微镜揭示的由黄粉虫（鞘翅目：拟步甲科）体内的尼氏簇虫（真簇虫目）产生的卵囊的表面结构。将配子囊置于15毫米的圆形盖玻片上使其开裂；然后将带有卵囊链的盖玻片直接安装在短柱上，无需进一步处理，并溅射镀上20纳米的金钯。使用日立3000N扫描电子显微镜在10 - 15千伏电压下进行扫描电子显微镜观察。卵囊保持了原始物种描述中所报道的特征形状，但在卵囊赤道中部隆起两侧的不同区域显示出高度、宽度和间距相对均匀的纵向脊。没有超微结构证据表明存在将卵囊连成链的外部包裹鞘。卵囊末端略有扩张，链本身呈扭曲状，相邻卵囊彼此略有偏移。本文现在提供了一组额外的结构特征，可能对簇虫系统学有用。
