Song Yong-Chun, Fu Si-Dong, Wen Xiao-Peng, Ma Zhen-Hua, Dang Cheng-Xue
Department of Oncology Surgery,The First Affiliated Hospital, Medical College, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, P. R. China.
Ai Zheng. 2007 Jul;26(7):709-14.
BACKGROUND & OBJECTIVE: BP1, a novel transcriptional factor, belongs to DLX family of homeobox genes. Recent researches showed that BP1 gene is correlated to genesis of breast cancer, but its correlation to cell cycle control factor has not been reported yet. This study was to observe the expression of BP1 in breast cancer, and to make clear its correlation to Cyclin D1.
The expression of BP1 and Cyclin D1 in 86 specimens of human breast cancer and 20 specimens of normal breast tissue (3 cm away from primary tumor) was detected by reverse transcription-polymerase chain reaction (RT-PCR). BP1 poly antibody was made and was certificated by Western blot. The expression of BP1 and Cyclin D1 in 86 specimens of human breast cancer were detected by immunohistochemistry; their correlation was analyzed.
The positive rate of BP1 mRNA was significanlty higher in breast cancer than in normal breast tissues (69.8% vs. 0, P < 0.001). The positive rate of Cyclin D1 mRNA was 64.0% in breast cancer. BP1 mRNA and Cyclin D1 mRNA were co-expressed in 52 specimens of breast cancer, and simultaneously negative in 23 specimens (P = 0.227); BP1 protein and Cyclin D1 protein were co-expressed in 43 specimens, and simultaneously negative in 31 specimens (P = 0.146).
BP1 gene is highly expressed in breast cancer. There is co-expression of Cyclin D1 and BP1 in breast cancer. BP1 gene may promote the genesis of breast cancer through regulating the expression of Cyclin D1.
BP1是一种新型转录因子，属于同源异型盒基因的DLX家族。近期研究表明，BP1基因与乳腺癌的发生相关，但其与细胞周期调控因子的相关性尚未见报道。本研究旨在观察BP1在乳腺癌中的表达，并明确其与细胞周期蛋白D1（Cyclin D1）的相关性。
采用逆转录-聚合酶链反应（RT-PCR）检测86例人乳腺癌标本及20例正常乳腺组织（距原发肿瘤3 cm）中BP1和Cyclin D1的表达。制备BP1多克隆抗体并通过蛋白质免疫印迹法进行鉴定。采用免疫组织化学法检测86例人乳腺癌标本中BP1和Cyclin D1的表达，并分析其相关性。
乳腺癌中BP1 mRNA的阳性率显著高于正常乳腺组织（69.8% 对0，P < 0.001）。乳腺癌中Cyclin D1 mRNA的阳性率为64.0%。52例乳腺癌标本中BP1 mRNA和Cyclin D1 mRNA共表达，23例标本同时为阴性（P = 0.227）；43例标本中BP1蛋白和Cyclin D1蛋白共表达，31例标本同时为阴性（P = 0.146）。
BP1基因在乳腺癌中高表达。乳腺癌中存在Cyclin D1与BP1的共表达。BP1基因可能通过调控Cyclin D1的表达促进乳腺癌的发生。
