Bayer S A, Altman J
Department of Biology, Indian-Purdue University, Indianapolis 46202.
Neuroscience. 1991;45(2):391-412. doi: 10.1016/0306-4522(91)90236-h.
Long-survival [3H]thymidine autoradiography was used to quantitatively determine the time of origin of neurons in the endopiriform nucleus and the claustrum in rats killed on postnatal day 60 after their dams received two consecutive daily injections of [3H]thymidine on embryonic day E13 and E14, E14 and E15, ... E21 and E22. The claustrum originates late, on E15 and E16, and has a strong gradient in the longitudinal direction, posterior (older) to anterior (younger). In contrast, the endopiriform nucleus originates early, on E14 and E15, and lacks a longitudinal gradient but has a strong one in the vertical direction, ventral (older) to dorsal (younger). Sequential-survival [3H]thymidine autoradiography was used to qualitatively determine the germinal sources and settling sites of endopiriform and claustral neurons in embryonic rats. The dams received a single injection of [3H]thymidine on either E14 (to heavily label older endopiriform neurons) or E16 (to heavily label younger claustral neurons) and were killed in sequential 24-h intervals. Neurons in the presumptive endopiriform nucleus settle within two to three days after their peak time of neurogenesis while those in the presumptive claustrum take approximately five days to settle after their peak. It is postulated that endopiriform neurons are generated in the palliostriatal ventricular angle, the neuroepithelium that forms a wedge between the primordia of the neocortex and the basal ganglia, and that claustral neurons are generated in the neocortical neuroepithelium. Divergent developmental patterns between the endopiriform nucleus and the claustrum support the anatomical evidence that these nuclei have different connections. Furthermore, neurogenetic gradients in the claustrum correlate with the pattern of anatomical connections between the claustrum and the neocortex.
在母鼠于胚胎期第13天和14天、14天和15天……21天和22天连续两天每日注射[3H]胸腺嘧啶核苷后，于出生后第60天处死大鼠，采用长期存活的[3H]胸腺嘧啶核苷放射自显影术定量测定大鼠内梨状核和屏状核神经元的起源时间。屏状核起源较晚，在胚胎期第15天和16天，且在纵向上有很强的梯度，从后（较老）到前（较年轻）。相比之下，内梨状核起源较早，在胚胎期第14天和15天，缺乏纵向梯度，但在垂直方向上有很强的梯度，从腹侧（较老）到背侧（较年轻）。采用连续存活的[3H]胸腺嘧啶核苷放射自显影术定性测定胚胎大鼠内梨状核和屏状核神经元的生发来源和定居部位。母鼠在胚胎期第14天（对内梨状核较老的神经元进行大量标记）或第16天（对屏状核较年轻的神经元进行大量标记）接受一次[3H]胸腺嘧啶核苷注射，并按连续24小时间隔处死。推测的内梨状核中的神经元在其神经发生高峰期后的两到三天内定居，而推测的屏状核中的神经元在其高峰期后大约需要五天才能定居。据推测，内梨状核神经元在苍白球纹状体室角产生，该神经上皮在新皮层和基底神经节原基之间形成一个楔形，而屏状核神经元在新皮层神经上皮中产生。内梨状核和屏状核之间不同的发育模式支持了这些核具有不同连接的解剖学证据。此外，屏状核中的神经发生梯度与屏状核和新皮层之间的解剖连接模式相关。
