Ratcliffe Lucy V, Rutten Frank J M, Barrett David A, Whitmore Terry, Seymour David, Greenwood Claire, Aranda-Gonzalvo Yolanda, Robinson Steven, McCoustra Martin
Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, University Park, Nottingham, NG7 2RD, United Kingdom.
Anal Chem. 2007 Aug 15;79(16):6094-101. doi: 10.1021/ac070109q. Epub 2007 Jul 12.
A novel plasma-assisted desorption/ionization (PADI) method that can be coupled with atmospheric pressure sampling mass spectrometry to yield mass spectral information under ambient conditions of pressure and humidity from a range of surfaces without the requirement for sample preparation or additives is reported. PADI is carried out by generating a nonthermal plasma which interacts directly with the surface of the analyte. Desorption and ionization then occur at the surface, and ions are sampled by the mass spectrometer. The PADI technique is demonstrated and compared with desorption electrospray ionization (DESI) for the detection of active ingredients in a range of over-the-counter and prescription pharmaceutical formulations, including nonsterodial anti-inflammatory drugs (mefenamic acid, Ibugel, and ibuprofen), analgesics (paracetamol, Anadin Extra), and Beecham's "all in one" cold and flu remedy. PADI has also been successfully applied to the analysis of nicotine in tobacco and thiosulfates in garlic. PADI experiments have been performed using a prototype source interfaced with a Waters Platform LCZ single-quadrupole mass spectrometer with limited modifications and a Hiden Analytical HPR-60 molecular beam mass spectrometer (MBMS). The ability of PADI to rapidly detect active ingredients in pharmaceuticals without the need for prior sample preparation, solvents, or exposed high voltages demonstrates the potential of the technique for high-throughput screening in a pharmaceutical or forensic environment.
报道了一种新型等离子体辅助解吸/电离（PADI）方法，该方法可与大气压采样质谱联用，在压力和湿度的环境条件下，从一系列表面获取质谱信息，无需样品制备或添加物。PADI通过产生与分析物表面直接相互作用的非热等离子体来进行。然后在表面发生解吸和电离，离子由质谱仪采样。展示了PADI技术，并将其与解吸电喷雾电离（DESI）进行比较，用于检测一系列非处方和处方药物制剂中的活性成分，包括非甾体抗炎药（甲芬那酸、伊布凝胶和布洛芬）、镇痛药（对乙酰氨基酚、安乃近Extra）以及比彻姆的“一体化”感冒流感药。PADI还成功应用于烟草中尼古丁和大蒜中硫代硫酸盐的分析。使用与沃特世Platform LCZ单四极杆质谱仪接口的原型源（经过有限修改）和Hiden Analytical HPR - 60分子束质谱仪（MBMS）进行了PADI实验。PADI无需事先样品制备、溶剂或暴露高压就能快速检测药物中的活性成分，这证明了该技术在制药或法医环境中进行高通量筛选的潜力。
